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Literature summary for 2.7.1.6 extracted from

  • Chu, X.; Li, N.; Liu, X.; Li, D.
    Functional studies of rat galactokinase (2009), J. Biotechnol., 141, 142-146.
    View publication on PubMed
Search for more literature for 2.7.1.6

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged, expressed in Escherichia coli BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D186A residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme Rattus norvegicus
D186E residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme Rattus norvegicus
D186N residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme Rattus norvegicus
R37A residue R37 interacts with galactose Rattus norvegicus
Y109F residue Y109interacts with ATP Rattus norvegicus
Y109H residue Y109interacts with ATP Rattus norvegicus
Y236A residue Y236 interacts with galactose Rattus norvegicus
Y236F residue Y236 interacts with galactose Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
amikacin
-
 Rattus norvegicus
AMP
-
 Rattus norvegicus
bromo alpha-D-galactopyranoside
-
 Rattus norvegicus
fluoro alpha-D-galactopyranoside
-
 Rattus norvegicus
galactose-1-phosphate
-
 Rattus norvegicus
genistein
-
 Rattus norvegicus
gentamycin
-
 Rattus norvegicus
kanamycin A
-
 Rattus norvegicus
neomycin
-
 Rattus norvegicus
paromycin
-
 Rattus norvegicus
quercetin
-
 Rattus norvegicus
tobramycin
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0948
-
 ATP wild-type protein Rattus norvegicus
0.136
-
 ATP Y236F mutant protein Rattus norvegicus
0.152
-
 ATP R37A mutant protein Rattus norvegicus
0.698
-
 alpha-D-galactose wild-type protein Rattus norvegicus
0.755
-
 ATP Y109F mutant protein Rattus norvegicus
0.784
-
 alpha-D-galactose R37A mutant protein Rattus norvegicus
0.823
-
 alpha-D-galactose Y109F mutant protein Rattus norvegicus
0.986
-
 alpha-D-galactose Y236F mutant protein Rattus norvegicus
1.118
-
 alpha-D-galactose Y109H mutant protein Rattus norvegicus
3.83
-
 ATP Y109H mutant protein Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000 Da, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
 R37A mutant protein, substrate: galactose Rattus norvegicus
0.14
-
 Y109F mutant protein, substrate: galactose Rattus norvegicus
0.17
-
 Y236F mutant protein, substrate: galactose Rattus norvegicus
0.91
-
 wild-type protein, substrate: galactose Rattus norvegicus
1.14
-
 Y109H mutant protein, substrate: galactose Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-2-deoxy-D-galactose + ATP
-
 Rattus norvegicus 2-amino-2-deoxy-D-galactose 1-phosphate + ADP
-
 ?
2-deoxy-D-galactose + ATP
-
 Rattus norvegicus 2-deoxy-D-galactose 1-phosphate + ADP
-
 ?
3-deoxy-D-galactose + ATP
-
 Rattus norvegicus ?
-
 ?
alpha-D-galactose + ATP
-
 Rattus norvegicus alpha-D-galactose 1-phosphate + ADP
-
 ?
D-fucose + ATP
-
 Rattus norvegicus ?
-
 ?
D-galactosamine + ATP
-
 Rattus norvegicus ?
-
 ?
D-talose + ATP
-
 Rattus norvegicus alpha-D-talose 1-phosphate + ADP
-
 ?

Subunits

Subunits Comment Organism
? x * 43000 Da, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
galactokinase
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 alpha-D-galactose R37A mutant protein Rattus norvegicus
0.1
-
 alpha-D-galactose Y109F mutant protein Rattus norvegicus
0.1
-
 alpha-D-galactose Y109H mutant protein Rattus norvegicus
0.12
-
 alpha-D-galactose Y236F mutant protein Rattus norvegicus
0.65
-
 alpha-D-galactose wild-type protein Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0225
-
 quercetin
-
 Rattus norvegicus
0.0739
-
 genistein
-
 Rattus norvegicus
10.2
-
 AMP
-
 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.86
-
-
 Rattus norvegicus fluoro alpha-D-galactopyranoside
4.4
-
-
 Rattus norvegicus bromo alpha-D-galactopyranoside
7.2
-
-
 Rattus norvegicus tobramycin
16.8
-
-
 Rattus norvegicus Galactose 1-phosphate
20
-
-
 Rattus norvegicus kanamycin A
30
-
-
 Rattus norvegicus neomycin
36.6
-
-
 Rattus norvegicus gentamycin
49.9
-
-
 Rattus norvegicus amikacin
67.3
-
-
 Rattus norvegicus paromycin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00057
-
 D-fucose Y236F mutant protein Rattus norvegicus
0.00062
-
 D-fucose R37A mutant protein Rattus norvegicus
0.0022
-
 2-amino-2-deoxy-D-galactose R37A mutant protein Rattus norvegicus
0.0027
-
 2-amino-2-deoxy-D-galactose Y236F mutant protein Rattus norvegicus
0.0047
-
 D-fucose wild-type protein Rattus norvegicus
0.0052
-
 D-talose R37A mutant protein Rattus norvegicus
0.012
-
 D-fucose Y109F mutant protein Rattus norvegicus
0.013
-
 D-galactosamine Y236F mutant protein Rattus norvegicus
0.019
-
 2-deoxy-D-galactose R37A mutant protein Rattus norvegicus
0.019
-
 D-talose Y109H mutant protein Rattus norvegicus
0.024
-
 3-Deoxy-D-galactose Y109F mutant protein Rattus norvegicus
0.026
-
 2-amino-2-deoxy-D-galactose Y109F mutant protein Rattus norvegicus
0.026
-
 D-fucose Y109H mutant protein Rattus norvegicus
0.026
-
 2-deoxy-D-galactose Y109H mutant protein Rattus norvegicus
0.028
-
 3-Deoxy-D-galactose Y109H mutant protein Rattus norvegicus
0.031
-
 D-talose Y109F mutant protein Rattus norvegicus
0.032
-
 2-amino-2-deoxy-D-galactose wild-type protein Rattus norvegicus
0.037
-
 3-Deoxy-D-galactose R37A mutant protein Rattus norvegicus
0.037
-
 2-deoxy-D-galactose Y109F mutant protein Rattus norvegicus
0.041
-
 3-Deoxy-D-galactose Y236F mutant protein Rattus norvegicus
0.047
-
 D-galactosamine Y109H mutant protein Rattus norvegicus
0.047
-
 2-deoxy-D-galactose Y236F mutant protein Rattus norvegicus
0.054
-
 3-Deoxy-D-galactose wild-type protein Rattus norvegicus
0.057
-
 D-talose Y236F mutant protein Rattus norvegicus
0.059
-
 2-amino-2-deoxy-D-galactose Y109H mutant protein Rattus norvegicus
0.06
-
 D-galactosamine Y109F mutant protein Rattus norvegicus
0.064
-
 alpha-D-galactose R37A mutant protein Rattus norvegicus
0.066
-
 D-galactosamine R37A mutant protein Rattus norvegicus
0.089
-
 alpha-D-galactose Y109H mutant protein Rattus norvegicus
0.12
-
 alpha-D-galactose Y109F mutant protein Rattus norvegicus
0.122
-
 alpha-D-galactose Y236F mutant protein Rattus norvegicus
0.14
-
 D-talose wild-type protein Rattus norvegicus
0.237
-
 2-deoxy-D-galactose wild-type protein Rattus norvegicus
0.615
-
 D-galactosamine wild-type protein Rattus norvegicus
0.93
-
 alpha-D-galactose wild-type protein Rattus norvegicus