Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.6 extracted from

  • Sellick, C.A.; Reece, R.J.
    Contribution of amino acid side chains to sugar binding specificity in a galactokinase, Gal1p, and a transcriptional inducer, Gal3p (2006), J. Biol. Chem., 281, 17150-17155.
    View publication on PubMed
Search for more literature for 2.7.1.6

Protein Variants

Protein Variants Comment Organism
D62A abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
D62A/Y274A shows no sugar kinase activity Saccharomyces cerevisiae
D62C shows no sugar kinase activity Saccharomyces cerevisiae
D62E shows no detectable sugar kinase activity Saccharomyces cerevisiae
D62F abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
D62G shows no sugar kinase activity Saccharomyces cerevisiae
D62H abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
D62I shows no sugar kinase activity Saccharomyces cerevisiae
D62K shows no detectable sugar kinase activity Saccharomyces cerevisiae
D62L abolishes all detectable galactokinase activity but retains the ability to use D-glucose as a substrate Saccharomyces cerevisiae
D62M shows no sugar kinase activity Saccharomyces cerevisiae
D62N shows no detectable sugar kinase activity Saccharomyces cerevisiae
D62P shows no sugar kinase activity Saccharomyces cerevisiae
D62Q shows no sugar kinase activity Saccharomyces cerevisiae
D62R shows no sugar kinase activity Saccharomyces cerevisiae
D62S shows no sugar kinase activity Saccharomyces cerevisiae
D62T shows no sugar kinase activity Saccharomyces cerevisiae
D62V shows no sugar kinase activity Saccharomyces cerevisiae
D62W shows no sugar kinase activity Saccharomyces cerevisiae
D62Y shows no sugar kinase activity Saccharomyces cerevisiae
R53A/D217A shows no sugar kinase activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 D-glucose mutant enzyme D62F, at 30°C Saccharomyces cerevisiae
0.046
-
 ATP in the presence of 2-deoxy-D-galactose as a substrate, at 30°C, wild type enzyme Saccharomyces cerevisiae
0.057
-
 ATP in the presence of D-galactose as a substrate, wild type enzyme Saccharomyces cerevisiae
0.107
-
 D-glucose mutant enzyme D62A, at 30°C Saccharomyces cerevisiae
0.137
-
 D-glucose mutant enzyme D62L, at 30°C Saccharomyces cerevisiae
0.148
-
 D-glucose mutant enzyme D62H, at 30°C Saccharomyces cerevisiae
0.225
-
 ATP in the presence of D-glucose as a substrate, at 30°C, wild type enzyme Saccharomyces cerevisiae
0.225
-
 D-glucose wild type enzyme, at 30°C Saccharomyces cerevisiae
0.271
-
 ATP mutant enzyme D62L, at 30°C Saccharomyces cerevisiae
0.296
-
 ATP mutant enzyme D62F, at 30°C Saccharomyces cerevisiae
0.34
-
 ATP mutant enzyme D62A, at 30°C Saccharomyces cerevisiae
0.371
-
 ATP mutant enzyme D62H, at 30°C Saccharomyces cerevisiae
0.539
-
 D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae
29.21
-
 2-deoxy-D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae mc2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic acid-agarose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-galactose
-
 Saccharomyces cerevisiae ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
 ?
ATP + 2-deoxy-D-galactose
-
 Saccharomyces cerevisiae mc2 ADP + 2-deoxy-alpha-D-galactose 1-phosphate
-
 ?
ATP + D-galactose
-
 Saccharomyces cerevisiae ADP + alpha-D-galactose 1-phosphate
-
 ?
ATP + D-galactose
-
 Saccharomyces cerevisiae mc2 ADP + alpha-D-galactose 1-phosphate
-
 ?
ATP + D-glucose
-
 Saccharomyces cerevisiae ADP + alpha-D-glucose 1-phosphate
-
 ?
ATP + D-glucose
-
 Saccharomyces cerevisiae mc2 ADP + alpha-D-glucose 1-phosphate
-
 ?
additional information L-arabinose, D-fucose, L-glucose, and D-talose are no substrates Saccharomyces cerevisiae ?
-
 ?
additional information L-arabinose, D-fucose, L-glucose, and D-talose are no substrates Saccharomyces cerevisiae mc2 ?
-
 ?

Synonyms

Synonyms Comment Organism
Gal1p
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 D-glucose mutant enzyme D62F, at 30°C Saccharomyces cerevisiae
0.019
-
 D-glucose mutant enzyme D62A, at 30°C Saccharomyces cerevisiae
0.034
-
 D-glucose wild type enzyme, at 30°C Saccharomyces cerevisiae
0.037
-
 D-glucose mutant enzyme D62L, at 30°C Saccharomyces cerevisiae
0.067
-
 D-glucose mutant enzyme D62H, at 30°C Saccharomyces cerevisiae
3.13
-
 2-deoxy-D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae
4.01
-
 2-deoxy-D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae
22.3
-
 D-galactose wild type enzyme, at 30°C Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae