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Literature summary for 2.7.1.6 extracted from

  • Yang, J.; Fu, X.; Liao, J.; Liu, L.; Thorson, J.S.
    Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization (2005), Chem. Biol., 12, 657-664.
    View publication on PubMed
Search for more literature for 2.7.1.6

Application

Application Comment Organism
synthesis enzyme mutant Y371H/M173L, substrate range is much wider than wild-type, generation of unnatural sugar 1-phosphates Escherichia coli

Protein Variants

Protein Variants Comment Organism
M173L moderate activity on D-glucose, wider substrate specificity than wild-type Escherichia coli
M173L/Y371H acts on additional substrates such as 4-azido-4-deoxy-D-galactose, 6-ido-6-deoxy-D-galactose, 6-chloro-6-deoxy-D-galactose, 6-bromo-6-deoxy-D-galactose, 4-deoxy-D-galactose, 6-thio-6-deoxy-D-galactose, 6-thio-6-deoxy-D-glucose, 6-azido-6-deoxy-D-glucose Escherichia coli
Y371H wider substrate specificity than wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.09
-
 D-galactose wild-type, 37°C Escherichia coli
2.57
-
 D-glucose mutant M173L, 37°C Escherichia coli
4.02
-
 D-glucose mutant M173L/Y371H, 37°C Escherichia coli
4.59
-
 D-galactose mutant M173L/Y371H, 37°C Escherichia coli
5.62
-
 D-galactose mutant Y371H, 37°C Escherichia coli
5.87
-
 D-galactose mutant M173L, 37°C Escherichia coli
6.04
-
 L-altrose mutant Y371H, 37°C Escherichia coli
6.28
-
 L-altrose mutant M173L/Y371H, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-galactose
-
 Escherichia coli ADP + alpha-D-galactose 1-phosphate
-
 ?
ATP + D-glucose
-
 Escherichia coli ADP + alpha-D-glucose 1-phosphate
-
 ?
ATP + L-altrose
-
 Escherichia coli ADP + L-altrose 1-phosphate
-
 ?