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Literature summary for 2.7.1.6 extracted from
- Purification and kinetic properties of galactokinase from human placenta (1972), Arch. Biochem. Biophys., 150, 191-198.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol protects the enzyme better than 2-mercaptoethanol against denaturation on storage at 4°C | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-galactose 1-phosphate | noncompetitive with respect to galactose | Homo sapiens |  |
| D-galactose 1-phosphate | - |
Homo sapiens | |
| Hg2+ | - |
Homo sapiens | |
| NEM | 1.0 mM, 41% inhibition | Homo sapiens | |
| PCMB | 1.0 mM, complete inhibition | Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
ATP | 37°C | Homo sapiens | |
| 0.25 | - |
D-galactose | 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | absolute requirement for a divalent cation, Ca2+ can be partially replaced by Co2+ | Homo sapiens | |
| Co2+ | absolute requirement for a divalent cation, Mg2+ can be partially replaced by Co2+ | Homo sapiens | |
| Mg2+ | absolute requirement for a divalent cation, maximally active in presence of 5 mM Mg2+ | Homo sapiens | |
| Mn2+ | absolute requirement for a divalent cation, Mg2+ can be partially replaced by Co2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.15 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-galactose | - |
Homo sapiens | ADP + alpha-D-galactose 1-phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
10 min, no loss of activity | Homo sapiens |
| 50 | - |
10 min, 25% loss of activity | Homo sapiens |
| 60 | - |
10 min, 60% loss of activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
D-galactose 1-phosphate | 37°C | Homo sapiens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing | - |
5.8 |
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