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Literature summary for 2.7.1.6 extracted from

  • Lee, L.; Kimura, A.; Tochikura, T.
    Purification and properties of galactokinase from Bifidobacterium bifidum (1977), J. Ferment. Technol., 55, 19-26.
No PubMed abstract available
Search for more literature for 2.7.1.6

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 0.5 mM HgCl2, complete inhibition, activity is completely restored by 1 mM dithiothreitol Bifidobacterium bifidum
NEM 2.0 mM, 12% inhibition Bifidobacterium bifidum
PCMB 0.1 mM, complete inhibition, activity is completely restored by 1 mM dithiothreitol Bifidobacterium bifidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.167
-
 ATP pH 7.5, 25°C Bifidobacterium bifidum
1.11
-
 D-galactose pH 7.5, 25°C Bifidobacterium bifidum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, half-maximal velocity is 2.2 mM Bifidobacterium bifidum
Mn2+
-
 Bifidobacterium bifidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtration Bifidobacterium bifidum
48000
-
 1 * 48000, SDS-PAGE Bifidobacterium bifidum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bifidobacterium bifidum

Storage Stability

Storage Stability Organism
-20°C, 50% loss of activity after 1 months Bifidobacterium bifidum
4°C, potassium phosphate buffer, pH 7.5, more than 80% of the initial activity remains after 1 week Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-galactose very strict substrate specificity for galactose and ATP Bifidobacterium bifidum ADP + alpha-D-galactose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48000, SDS-PAGE Bifidobacterium bifidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bifidobacterium bifidum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 30% of maximal activity, 60°C: about 40% of maximal activity Bifidobacterium bifidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, 20% loss of activity Bifidobacterium bifidum
90
-
 10 min, 10 mM dithiothreitol, stable Bifidobacterium bifidum
95
-
 20 min, 2 mM dithiothreitol, 60% loss of activity Bifidobacterium bifidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bifidobacterium bifidum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 60% of maximal activity, pH 8.0: about 70% of maximal activity, potassium phosphate buffer Bifidobacterium bifidum