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Literature summary for 2.7.1.6 extracted from
- Purification and properties of galactokinase from Tetrahymena thermophila (1982), Biochim. Biophys. Acta, 717, 76-85.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine-5'-[gamma-thio]triphosphate | - |
Tetrahymena thermophila |  |
| ADP | - |
Tetrahymena thermophila | |
| Cd2+ | - |
Tetrahymena thermophila | |
| Cu2+ | - |
Tetrahymena thermophila | |
| Zn2+ | - |
Tetrahymena thermophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
ATP | pH 7.5, 37°C | Tetrahymena thermophila | |
| 0.6 | - |
D-galactose | pH 7.5, 37°C | Tetrahymena thermophila | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required, in the order of decreasing efficiency: Mg2+, Co2+, Mn2+, Fe2+ | Tetrahymena thermophila | |
| Fe2+ | divalent cation required, in the order of decreasing efficiency: Mg2+, Co2+, Mn2+, Fe2+ | Tetrahymena thermophila | |
| Mg2+ | divalent cation required, in the order of decreasing efficiency: Mg2+, Co2+, Mn2+, Fe2+ | Tetrahymena thermophila | |
| Mn2+ | divalent cation required, in the order of decreasing efficiency: Mg2+, Co2+, Mn2+, Fe2+ | Tetrahymena thermophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
2 * 28000, SDS-PAGE | Tetrahymena thermophila |
| 50000 | 55000 | gel filtration | Tetrahymena thermophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena thermophila | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tetrahymena thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-dATP + D-galactose | - |
Tetrahymena thermophila | 2'-dADP + alpha-D-galactose 1-phosphate | - |
? | |
| 3'-dATP + D-galactose | - |
Tetrahymena thermophila | 3'-dADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + 2-deoxy-D-galactose | - |
Tetrahymena thermophila | ADP + 2-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactosamine | - |
Tetrahymena thermophila | ADP + D-galactosamine 1-phosphate | - |
? | |
| ATP + D-galactose | - |
Tetrahymena thermophila | ADP + alpha-D-galactose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 28000, SDS-PAGE | Tetrahymena thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | - |
- |
Tetrahymena thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Tetrahymena thermophila |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 8.7 | pH 6.7: about 45% of maximal activity, pH 8.7: about 35% of maximal activity | Tetrahymena thermophila |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Tetrahymena thermophila | isoelectric focusing | - |
5.1 |
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