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Literature summary for 2.7.1.6 extracted from

  • Lee, L.J.; Kinoshita, S.; Kumagai, H.; Tochikura, T.
    Galactokinase of Bifidobacterium bifidum (1980), Agric. Biol. Chem., 44, 2961-2966.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Bifidobacterium bifidum

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive with respect to ATP, uncompetitive with respect to galactose Bifidobacterium bifidum
Hg2+ 0.5 mM HgCl2, complete inhibition, activity is completely restored by 1 mM dithiothreitol Bifidobacterium bifidum
PCMB 0.1 mM, complete inhibition, activity is completely restored by 1 mM dithiothreitol Bifidobacterium bifidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
ATP pH 8.7, 25°C Bifidobacterium bifidum
1.85
-
D-galactose pH 8.7, 25°C Bifidobacterium bifidum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace for Mg2+ Bifidobacterium bifidum
Mg2+ required Bifidobacterium bifidum
Mn2+ can partially replace for Mg2+ Bifidobacterium bifidum
Zn2+ can partially replace for Mg2+ Bifidobacterium bifidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
gel filtration, calculation from sedimentation and diffusion data Bifidobacterium bifidum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bifidobacterium bifidum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
318
-
-
Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-galactose very strict substrate specificity for galactose and ATP Bifidobacterium bifidum ADP + alpha-D-galactose 1-phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 7.5, 10 min, stable up to Bifidobacterium bifidum
60
-
10 min, 50% loss of activity Bifidobacterium bifidum
70
-
10 min, complete loss of activity without dithiothreitol Bifidobacterium bifidum
90
-
10 min, in presence of 2 mM dithiothreitol or dithioerythritol, 50% loss of activity Bifidobacterium bifidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Bifidobacterium bifidum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
very labile below Bifidobacterium bifidum
4.5
-
37°C, 10 min, about 20% loss of activity Bifidobacterium bifidum
6
-
stable above Bifidobacterium bifidum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6
-
ADP pH 8.7, 25°C Bifidobacterium bifidum