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Literature summary for 2.7.1.6 extracted from
- Purification and properties of galactokinase from Saccharomyces cerevisiae (1977), J. Biol. Chem., 252, 1162-1166.
General Stability
| General Stability | Organism |
|---|---|
| overnight dialysis at pH above 9.2 or below pH 5.5 results in loss of all enzymatic activity | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
ATP | pH 8.0, 30°C | Saccharomyces cerevisiae |  |
| 0.6 | - |
D-galactose | pH 8.0, 30°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
equilibrium sedimentation, sucrose density gradient centrifugation | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38.3 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, less than 20% loss of activity after 6 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-galactose | - |
Saccharomyces cerevisiae | ADP + alpha-D-galactose 1-phosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 55.8 | - |
ATP | pH 8.0, 30°C | Saccharomyces cerevisiae | |
| 55.8 | - |
D-galactose | pH 8.0, 30°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH-optimum at pH 8 with a sharp drop in activity above pH 9.0 and a more gradual drop below pH 7.0 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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