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Literature summary for 2.7.1.6 extracted from
- Galactokinase from pig liver (1975), Methods Enzymol., 42C, 43-47.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 5 mM, 2-4fold stimulation | Sus scrofa |  |
| cysteine | 5 mM, 2-4fold stimulation | Sus scrofa | |
| dithiothreitol | 5 mM, 2-4fold stimulation | Sus scrofa | |
| glutathione | 5 mM, 2-4fold stimulation | Sus scrofa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive inhibitor of MgATP2- | Sus scrofa | |
| alpha-D-galactose 1-phosphate | noncompetitive inhibitor of galactose | Sus scrofa | |
| galactose | above 1 mM | Sus scrofa | |
| Mg2+ | free Mg2+ is a competitive inhibitor of MgATP2- | Sus scrofa | |
| PCMB | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
ATP | pH 7.8 | Sus scrofa | |
| 0.17 | - |
ATP | ATP in form of MgATP2- | Sus scrofa | |
| 0.58 | - |
D-galactose | pH 7.8 | Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | divalent cations activity in the order of decreasing efficiency: Mn2+, Mg2+, Ca2+, Fe2+ | Sus scrofa | |
| Co2+ | divalent cations activate in the order of decreasing efficiency: Mn2+, Mg2+, Ca2+, Fe2+ | Sus scrofa | |
| Fe2+ | divalent cations activity in the order of decreasing efficiency: Mn2+, Mg2+, Ca2+, Fe2+ | Sus scrofa | |
| Mg2+ | divalent cations activate in the order of decreasing efficiency: Mn2+, Mg2+, Ca2+, Fe2+ | Sus scrofa | |
| Mn2+ | divalent cations activate in the order of decreasing efficiency: Mn2+, Mg2+, Ca2+, Fe2+ | Sus scrofa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | of 2-week-old piglets. Activities in livers of fetal or suckling mammals and decreases at weaning so that the activity in adults is between 10 and 20% of the maximum attained during development | Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, galactokinase suspension in saturated (NH4)2SO4 retains at least 80% of its activity after 1 week | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-galactose | - |
Sus scrofa | ADP + 2-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | - |
Sus scrofa | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactosamine | - |
Sus scrofa | ADP + D-galactosamine 1-phosphate | - |
? | |
| ATP + D-galactose | - |
Sus scrofa | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| deoxy-ATP + D-galactose | - |
Sus scrofa | deoxyADP + alpha-D-galactose 1-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | in Tris or triethanolamine buffer | Sus scrofa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
ADP | - |
Sus scrofa | |
| 25 | - |
Mg2+ | - |
Sus scrofa | |
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