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Literature summary for 2.7.1.6 extracted from

  • Talbot, B.; Thirion, J.P.
    Isolation, purification and partial characterization of galactokinase from Chinese hamster liver (1982), Int. J. Biochem., 14, 719-726.
    View publication on PubMed

General Stability

General Stability Organism
the enzyme is stabilized during the purification procedure by the inclusion of 10% glycerol, 1 mM phosphate and 20 mM 2-mercaptoethanol in all buffer solutions Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.116
-
D-galactose
-
Cricetulus griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two forms of galactokinase, one of which is purified to homogeneity Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-galactose
-
Cricetulus griseus ADP + alpha-D-galactose 1-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Cricetulus griseus