Cloned (Comment) | Organism |
---|---|
6 point mutations in cysteine residues, expression in Escherichia coli BL21 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C131S | strongly decreased activity, very low affinity for N-acetyl-D-glucosamine | Mus musculus |
C143S | strongly decreased activity, very low affinity for ATP | Mus musculus |
C211S | slightly decreased activity | Mus musculus |
C217S | decreased activity, low affinity for N-acetyl-D-glucosamine | Mus musculus |
C268S | decreased activity | Mus musculus |
C45S | very slightly decreased activity, no function in substrate binding | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for both substrates tested for wild type and all mutant enzymes | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
SDS-PAGE, wild type protein | Mus musculus |
42000 | - |
SDS-PAGE, from E. coli | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Mus musculus | first enzyme of N-acetyl-D-glucosamine salvage pathway | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from E. coli | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | - |
Mus musculus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | first enzyme of N-acetyl-D-glucosamine salvage pathway | Mus musculus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |