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Literature summary for 2.7.1.59 extracted from

  • Allen, M.B.; Walker, D.G.
    The isolation and preliminary characterization of N-acetyl-D-glucosamine kinase from rat kidney and liver (1980), Biochem. J., 185, 565-575.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
SDS-PAGE, second band at 90000 Da shows no activity Rattus norvegicus
80000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Rattus norvegicus
-
ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39
-
purified enzyme from liver, 32°C, pH 7.5 Rattus norvegicus
42
-
purified enzyme from kidney, 32°C, pH 7.5 Rattus norvegicus

Storage Stability

Storage Stability Organism
-10°C, several months, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose very poor substrate, 4.5% of the rate with N-acetyl-D-glucosamine Rattus norvegicus ADP + D-glucose 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine
-
Rattus norvegicus ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ATP + N-acetyl-D-mannosamine 78% of the rate with N-acetyl-D-glucosamine Rattus norvegicus ADP + N-acetyl-D-mannosamine 6-phosphate
-
?
additional information no activity with D-galactose and D-fructose, very low activities with N-acetyl-D-galactosamine: 7.6%, D-glucosamine: 11.2%, D-mannosamine: 2.7%, D-galactosamine: 2.1%, D-mannose: 4.5% and D-xylose: 2.1% Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE, gel filtration Rattus norvegicus