Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
SDS-PAGE, second band at 90000 Da shows no activity | Rattus norvegicus |
80000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Rattus norvegicus | - |
ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39 | - |
purified enzyme from liver, 32°C, pH 7.5 | Rattus norvegicus |
42 | - |
purified enzyme from kidney, 32°C, pH 7.5 | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-10°C, several months, no loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | very poor substrate, 4.5% of the rate with N-acetyl-D-glucosamine | Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | - |
Rattus norvegicus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | 78% of the rate with N-acetyl-D-glucosamine | Rattus norvegicus | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? | |
additional information | no activity with D-galactose and D-fructose, very low activities with N-acetyl-D-galactosamine: 7.6%, D-glucosamine: 11.2%, D-mannosamine: 2.7%, D-galactosamine: 2.1%, D-mannose: 4.5% and D-xylose: 2.1% | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE, gel filtration | Rattus norvegicus |