General Stability | Organism |
---|---|
unstable to dialysis and gel filtration, 0.013 mM substrate protects against inactivation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | irreversible | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
N-acetyl-D-glucosamine | 37°C, pH 8.0 | Homo sapiens | |
1.8 | - |
ATP | 37°C, pH 8.0 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation | Homo sapiens | |
Mg2+ | required | Homo sapiens | |
Mn2+ | can partially replace Mg2+ in activation | Homo sapiens | |
additional information | slight activities with Ni2+, Zn2+, Cu2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Homo sapiens | part of glycoprotein synthesis pathway | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastric mucosa | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.28 | - |
purified enzyme, 37°C, pH 8.0 | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 6 months | Homo sapiens |
4°C, stable for at least 3 days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | - |
Homo sapiens | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | part of glycoprotein synthesis pathway | Homo sapiens | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | roughly 50% of activity | Homo sapiens | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
84% loss of activity within 2 min, no protection with added substrate | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | - |
Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
50% loss of activity within 2 min, no protection with added substrate | Homo sapiens |