Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | reverses inhibition with p-hydroxymercuribenzoate | Streptococcus pyogenes |
General Stability | Organism |
---|---|
ammonium sulfate partially stabilizes against temperature inactivation | Streptococcus pyogenes |
dialysis at 4°C for 17 h against 0.005 M Tris buffer, pH 7.6, 92% loss of activity, 33% loss of activity in presence of 0.1 M N-acetyl-D-glucosamine or glucose, stable in presence of 0.2 M or 2.5 | Streptococcus pyogenes |
substrate at 0.2 M stabilizes, unstable in absence of substrate at 4°C | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | competitive with N-acetyl-D-glucosamine | Streptococcus pyogenes | |
N-acetyl-D-glucosamine | competitive with glucose | Streptococcus pyogenes | |
p-hydroxymercuribenzoate | no protection by substrate, protection but not reversal of inhibition by thioethanol, complete inhibition above 0.00125 mM, cysteine reverses inhibition | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
N-acetyl-D-glucosamine | 30°C, pH 7.4 | Streptococcus pyogenes | |
0.0011 | - |
D-glucose | 30°C, pH 7.4 | Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-D-glucosamine | Streptococcus pyogenes | - |
ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | Streptococcus pyogenes 523 | - |
ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
serotype 14 | - |
Streptococcus pyogenes 523 | - |
serotype 14 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus pyogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
250 | - |
purified enzyme, 30°C, pH 7.4 | Streptococcus pyogenes |
Storage Stability | Organism |
---|---|
-25°C, stable for several months | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | at equal rate than N-acetyl-D-glucosamine | Streptococcus pyogenes | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | at equal rate than N-acetyl-D-glucosamine | Streptococcus pyogenes 523 | ADP + D-glucose 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | - |
Streptococcus pyogenes | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | - |
Streptococcus pyogenes 523 | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
additional information | D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates | Streptococcus pyogenes | ? | - |
? | |
additional information | D-fructose, D-mannose, D-maltose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates | Streptococcus pyogenes 523 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
25% loss of activity within 1 min in presence of 13% ammonium sulfate, stable for 1 h in presence of ammonium sulfate and substrate | Streptococcus pyogenes |
70 | - |
complete inactivation with 1 min | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | strongly decreasing activity outside this range | Streptococcus pyogenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
N-acetyl-D-glucosamine | for reaction with glucose, 30°C, pH 7.4 | Streptococcus pyogenes | |
0.0018 | - |
D-glucose | for reaction with N-acetyl-D-glucosamine, 30°C, pH 7.4 | Streptococcus pyogenes |