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Literature summary for 2.7.1.58 extracted from

  • Taestensen, J.B.; Johnsen, U.; Reinhardt, A.; Ortjohann, M.; Schoenheit, P.
    D-galactose catabolism in archaea operation of the DeLey-Doudoroff pathway in Haloferax volcanii (2020), FEMS Microbiol. Lett., 367, fnaa029 .
    View publication on PubMed
Search for more literature for 2.7.1.58

Cloned(Commentary)

Cloned (Comment) Organism
gene kdgK1, recombinant overexpression in Haloferax volcanii strain H1209 Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 2-dehydro-3-deoxy-D-galactonate pH and temperature not specified in the publication Haloferax volcanii
0.3
-
 ATP pH and temperature not specified in the publication Haloferax volcanii
8.8
-
 2-dehydro-3-deoxy-D-gluconate pH and temperature not specified in the publication Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
194000
-
-
 Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii NCIMB 2012
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii JCM 8879
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii H26
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii DS2
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii DSM 3757
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii ATCC 29605
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii NBRC 14742
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii VKM B-1768
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GR05
-
-
Haloferax volcanii ATCC 29605 D4GR05
-
-
Haloferax volcanii DS2 D4GR05
-
-
Haloferax volcanii DSM 3757 D4GR05
-
-
Haloferax volcanii H26 D4GR05
-
-
Haloferax volcanii JCM 8879 D4GR05
-
-
Haloferax volcanii NBRC 14742 D4GR05
-
-
Haloferax volcanii NCIMB 2012 D4GR05
-
-
Haloferax volcanii VKM B-1768 D4GR05
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Haloferax volcanii strain H1209 Haloferax volcanii

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-galactose-grown cell
-
 Haloferax volcanii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.148
-
 purified recombinant enzyme, pH 8.0, 42°C Haloferax volcanii
148
-
 substrate: 2-dehydro-3-deoxy-D-galactonate, pH and temperature not specified in the publication Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii NCIMB 2012 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii JCM 8879 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii H26 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii DS2 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii DSM 3757 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii ATCC 29605 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii NBRC 14742 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii VKM B-1768 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii NCIMB 2012 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii JCM 8879 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii H26 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii DS2 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii DSM 3757 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii ATCC 29605 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii NBRC 14742 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii VKM B-1768 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 34000, calculated from sequence Haloferax volcanii

Synonyms

Synonyms Comment Organism
2-keto-3-deoxygalactonate kinase
-
 Haloferax volcanii
HVO A0328
-
 Haloferax volcanii
HVO_A0328
-
 Haloferax volcanii
KDGal kinase
-
 Haloferax volcanii
KDGK2 UniProt Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
 assay at Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Haloferax volcanii

Expression

Organism Comment Expression
Haloferax volcanii transcriptional regulator GacR is an activator of genes of the DeLey-Doudoroff pathway including the gene that encodes 2-keto-3-deoxygalactonate kinase up

General Information

General Information Comment Organism
malfunction growth studies with knock-out mutants indicate the functional involvement of galactose dehydrogenase, galactonate dehydratase, and KDPGal aldolase in D-galactose degradation Haloferax volcanii
malfunction the deletion mutant does not grow on D-galactose Haloferax volcanii
metabolism a cluster of genes encoding putative enzymes of the DeLey-Doudoroff pathway for D-galactose degradation including galactose dehydrogenase, galactonate dehydratase, 2-keto-3-deoxygalactonate kinase and 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase is involved in D-galactose degradation. The transcriptional regulator GacR is an activator of genes of the DeLey-Doudoroff pathway Haloferax volcanii
metabolism the enzyme is part of the De Ley-Doudoroff pathway Haloferax volcanii
physiological function the enzyme is required for D-galactose degradation via the DeLey-Doudoroff pathway in the domain of archaea Haloferax volcanii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 2-dehydro-3-deoxy-D-gluconate pH and temperature not specified in the publication Haloferax volcanii
432
-
 2-dehydro-3-deoxy-D-galactonate pH and temperature not specified in the publication Haloferax volcanii