Literature summary for 2.7.1.58 extracted from

Michalska, K.; Cuff, M.E.; Tesar, C.; Feldmann, B.; Joachimiak, A.
Structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae (2011), Acta Crystallogr. Sect. D, 67, 678-689.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21 (DE3) from plasmid pRK1037 harboring a fusion protein construct containing an N-terminal maltose-binding protein followed by a tobacco vein mottling virus protease cleavage site, a His6 -tag, a TEV protease cleavage site and the target protein 2-oxo-3-deoxygalactonate kinase	Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and mselenomethionine-labeled enzymes, sitting-drop vapor-diffusion, mixing of 0.0004 ml of 30 mg/m protein in 20 mM HEPES, pH 8.0, 250 mM NaCl, and 2 mM dithiothreitol, with 0.0004 ml of crystallization solution containing 0.1 M magnesium formate, 15%(w/v) PEG 3350, equilibration against 0.135 ml of crystallization solution, at 4°C or 24°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution	Klebsiella pneumoniae

Crystallization (Comment)

Organism

purified recombinant wild-type and mselenomethionine-labeled enzymes, sitting-drop vapor-diffusion, mixing of 0.0004 ml of 30 mg/m protein in 20 mM HEPES, pH 8.0, 250 mM NaCl, and 2 mM dithiothreitol, with 0.0004 ml of crystallization solution containing 0.1 M magnesium formate, 15%(w/v) PEG 3350, equilibration against 0.135 ml of crystallization solution, at 4°C or 24°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution

Klebsiella pneumoniae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31100	-	el filtration, recombinant detagged enzyme	Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 2-dehydro-3-deoxy-D-galactonate	Klebsiella pneumoniae	-	ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate	-	?
ATP + 2-dehydro-3-deoxy-D-galactonate	Klebsiella pneumoniae MGH 78578	-	ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate	-	?

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	A6TFZ6	subsp. pneumoniae	-
Klebsiella pneumoniae MGH 78578	A6TFZ6	subsp. pneumoniae	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally maltose-binding protein fused , His6 -tagged enzyme from Escherichia coli strain BL21 (DE3) by amylose and nickel affinity chromatographies interveined by the cleavage of the tags, to homogeneity, followed by ultrafiltration	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2-dehydro-3-deoxy-D-galactonate	-	Klebsiella pneumoniae	ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate	-	?
ATP + 2-dehydro-3-deoxy-D-galactonate	-	Klebsiella pneumoniae MGH 78578	ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate	-	?

Subunits

Subunits	Comment	Organism
More	the enzyme is composed of two alpha/betaa domains	Klebsiella pneumoniae

Synonyms

Synonyms	Comment	Organism
2-oxo-3-deoxy-D-galactonate kinase	-	Klebsiella pneumoniae
2-oxo-3-deoxygalactonate kinase	-	Klebsiella pneumoniae
KDGal kinase	-	Klebsiella pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Klebsiella pneumoniae

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the ASKHA (acetate and sugar kinases/hsc70/actin) family of phosphotransferases, ASKHA signature motifs in KDGal kinase, overview	Klebsiella pneumoniae
metabolism	the 2-oxo-3-deoxygalactonate kinase catalyzes the penultimate step of an alternative route of D-galactose assimilation, the so-called De Ley-Doudoroff pathway, in which D-galactose is metabolized into pyruvate and D-glyceraldehyde 3-phosphate in five consecutive reactions carried out by specific enzymes. The pathway involves the phosphorylation of 2-oxo-3-deoxygalactonate to 2-oxo-3-deoxygalactonate 6-phosphate catalyzed by the enzyme with ATP serving as a phosphoryl-group donor. Comparison with other D-galactose metabolic pathways, overview. 2-oxo-3-deoxygalactonate 6-phosphate decomposes into puruvate and D-glyceraldehyde 3-phosphate, both products are utilized by the glycolytic pathway	Klebsiella pneumoniae
additional information	the putative substrate-binding site is created mostly by the N-terminal domain, substrate binding includes the conserved Tyr78 from loop L6, His141 and Lys143 from L15, as well as Glu119 from the H4 helix, nucleotide-binding site structure, overview	Klebsiella pneumoniae