KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
D-Fructose 1-phosphate | pH 7.5, 25°C | Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 40 mM, 3fold increase of activity | Klebsiella aerogenes | |
Mg2+ | required for activity | Klebsiella aerogenes | |
Mg2+ | maximal activity at a Mg2+/ATP ratio of at least 2 | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
fructose 6-phosphate kinase-deficient mutant of a uracil auxotroph of strain PRL-R3 | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
-20°C, 100 mM (NH4)2SO4, pH 7.5, several months, no loss of activity | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | no activity with D-fructose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | no activity with D-glucose 6-phosphate, D-glucose 1-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | no activity with sorbose 1-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Klebsiella aerogenes |