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Literature summary for 2.7.1.56 extracted from

  • Hanson, T.E.; Anderson, R.L.
    D-fructose 1-phosphate kinase, a new enzyme instrumental in the metabolism of D-fructose (1966), J. Biol. Chem., 241, 1644-1645.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
D-Fructose 1-phosphate
-
Klebsiella aerogenes
0.8
-
ATP
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 1-phosphate Klebsiella aerogenes inducible enzyme ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 1-phosphate Klebsiella aerogenes involved in D-fructose metabolism ADP + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, ammonium sulfate, heat, Sephadex G-100, DEAE-cellulose, partially purified Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Klebsiella aerogenes
0.27
-
activity in crude extracts Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 1-phosphate no activity with D-fructose 6-phosphate Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 1-phosphate inducible enzyme Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 1-phosphate involved in D-fructose metabolism Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Klebsiella aerogenes