BRENDA - Enzyme Database
show all sequences of 2.7.1.56

D-fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties

Sapico, V.; Anderson, R.L.; J. Biol. Chem. 244, 6280-6288 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
additional Mg2+ reverses; at a Mg2+/ATP ratio below 2
Klebsiella aerogenes
citrate
-
Klebsiella aerogenes
D-fructose 1,6-bisphosphate
-
Klebsiella aerogenes
D-fructose 6-phosphate
-
Klebsiella aerogenes
GTP
at a Mg2+/GTP ratio below 2
Klebsiella aerogenes
ITP
at a Mg2+/ITP ratio below 2
Klebsiella aerogenes
additional information
not inhibited by ATP; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by nucleoside monophosphates or diphosphates, D-mannose 6-phosphate, D-fructose, L-fructose 1-phosphate; not inhibited by phosphate, AMP
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
ATP
-
Klebsiella aerogenes
0.75
-
D-Fructose 1-phosphate
-
Klebsiella aerogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for activity
Klebsiella aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Klebsiella aerogenes
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
wild-type strain PRL-R3
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
47
-
-
Klebsiella aerogenes
Storage Stability
Storage Stability
Organism
-20C, 100 mM (NH4)2SO4, pH 7.5, at least 8 months
Klebsiella aerogenes
-20C, crude enzyme extract prepared in water, at least 3 weeks
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose 1-phosphate
no activity with D-fructose 6-phosphate
640483
Klebsiella aerogenes
ADP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
ATP + D-fructose 1-phosphate
no activity with D-glucose 6-phosphate, D-glucose 1-phosphate
640483
Klebsiella aerogenes
ADP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
ATP + D-fructose 1-phosphate
specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate
640483
Klebsiella aerogenes
ADP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
CTP + D-fructose 1-phosphate
poor substrate
640483
Klebsiella aerogenes
CDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
GTP + D-fructose 1-phosphate
35% of activity with ATP
640483
Klebsiella aerogenes
GDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
ITP + D-fructose 1-phosphate
43% of activity with ATP
640483
Klebsiella aerogenes
IDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
TTP + D-fructose 1-phosphate
poor substrate
640483
Klebsiella aerogenes
TDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
UTP + D-fructose 1-phosphate
poor substrate
640483
Klebsiella aerogenes
UDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Klebsiella aerogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8.7
approx. 80% of maximal activity at pH 7. approx. half-maximal activity at pH 8.7
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
additional Mg2+ reverses; at a Mg2+/ATP ratio below 2
Klebsiella aerogenes
citrate
-
Klebsiella aerogenes
D-fructose 1,6-bisphosphate
-
Klebsiella aerogenes
D-fructose 6-phosphate
-
Klebsiella aerogenes
GTP
at a Mg2+/GTP ratio below 2
Klebsiella aerogenes
ITP
at a Mg2+/ITP ratio below 2
Klebsiella aerogenes
additional information
not inhibited by ATP; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by nucleoside monophosphates or diphosphates, D-mannose 6-phosphate, D-fructose, L-fructose 1-phosphate; not inhibited by phosphate, AMP
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
ATP
-
Klebsiella aerogenes
0.75
-
D-Fructose 1-phosphate
-
Klebsiella aerogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required for activity
Klebsiella aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Klebsiella aerogenes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
47
-
-
Klebsiella aerogenes
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 100 mM (NH4)2SO4, pH 7.5, at least 8 months
Klebsiella aerogenes
-20C, crude enzyme extract prepared in water, at least 3 weeks
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose 1-phosphate
no activity with D-fructose 6-phosphate
640483
Klebsiella aerogenes
ADP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
ATP + D-fructose 1-phosphate
no activity with D-glucose 6-phosphate, D-glucose 1-phosphate
640483
Klebsiella aerogenes
ADP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
ATP + D-fructose 1-phosphate
specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate
640483
Klebsiella aerogenes
ADP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
CTP + D-fructose 1-phosphate
poor substrate
640483
Klebsiella aerogenes
CDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
GTP + D-fructose 1-phosphate
35% of activity with ATP
640483
Klebsiella aerogenes
GDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
ITP + D-fructose 1-phosphate
43% of activity with ATP
640483
Klebsiella aerogenes
IDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
TTP + D-fructose 1-phosphate
poor substrate
640483
Klebsiella aerogenes
TDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
UTP + D-fructose 1-phosphate
poor substrate
640483
Klebsiella aerogenes
UDP + D-fructose 1,6-bisphosphate
-
640483
Klebsiella aerogenes
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Klebsiella aerogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8.7
approx. 80% of maximal activity at pH 7. approx. half-maximal activity at pH 8.7
Klebsiella aerogenes
Other publictions for EC 2.7.1.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738617
Chan
Yeast phosphofructokinase-1 su ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
J. Biol. Chem.
289
19448-19457
2014
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23
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-
-
-
-
-
-
-
-
1
1
-
-
-
737595
Funato
Nucleoredoxin regulates glucos ...
Mus musculus
Biochem. Biophys. Res. Commun.
440
737-742
2013
-
-
-
-
-
-
-
-
-
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1
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4
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4
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1
1
-
-
-
722543
Pickl
Fructose degradation in the ha ...
Haloferax volcanii, Haloferax volcanii H1209
J. Bacteriol.
194
3088-3097
2012
-
-
-
-
-
-
-
3
-
1
2
4
-
5
-
-
1
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4
1
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1
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3
-
1
2
4
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1
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-
4
1
-
-
-
-
1
-
-
-
1
1
1
1
2
2
684377
Su
Human H+ATPase a4 subunit muta ...
Homo sapiens
Am. J. Physiol. Renal Physiol.
295
F950-F958
2008
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-
-
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1
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1
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1
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687484
Peskov
Kinetic model of phosphofructo ...
Escherichia coli
J. Bioinform. Comput. Biol.
6
843-867
2008
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-
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1
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687740
Deng
Phosphorylation of Bad at Thr- ...
Mus musculus
J. Biol. Chem.
283
20754-20760
2008
-
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1
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1
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1
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1
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1
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1
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688049
Russell
Some characteristics of rabbit ...
Oryctolagus cuniculus
J. Enzyme Inhib. Med. Chem.
23
411-417
2008
-
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-
1
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1
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1
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1
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686728
Martinez-Costa
Chimeric phosphofructokinases ...
Homo sapiens
FEBS Lett.
581
3033-3038
2007
-
-
1
-
1
-
1
1
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1
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1
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2
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1
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661250
Hagopian
Fructose metabolizing enzymes ...
Mus musculus
Biochim. Biophys. Acta
1721
37-43
2005
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1
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3
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674240
Barriere
Fructose utilization in Lactoc ...
Lactococcus lactis, Lactococcus lactis JIM8240
J. Bacteriol.
187
3752-3761
2005
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5
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1
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2
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641686
Veiga-da-Cunha
Overexpression and purificatio ...
Escherichia coli
Protein Expr. Purif.
19
48-52
2000
-
-
1
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4
-
1
1
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2
-
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1
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1
1
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1
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1
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1
1
1
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727057
Rangaswamy
Characterization of 1-phosphof ...
Haloarcula vallismortis, Haloarcula vallismortis ATCC 34679
Biochim. Biophys. Acta
1201
106-112
1994
1
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6
2
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6
1
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5
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1
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10
1
1
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1
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1
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6
1
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6
1
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10
1
1
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1
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1
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641683
Buschmeier
-
Purification and properties of ...
Escherichia coli
FEMS Microbiol. Lett.
29
231-235
1985
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4
2
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2
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1
1
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2
2
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2
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1
1
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2
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641685
Bang
Properties of 1-phosphofructok ...
Pseudomonas putida
Can. J. Microbiol.
23
721-725
1977
1
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4
3
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1
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1
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1
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1
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1
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1
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640495
Baumann
Catabolism of D-fructose and D ...
Oceanimonas doudoroffii
Arch. Microbiol.
105
241-248
1975
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6
2
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5
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2
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1
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1
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1
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6
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2
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5
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1
2
3
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1
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1
1
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641680
Anderson
D-fructose-1-phosphate kinase ...
Klebsiella aerogenes
Methods Enzymol.
42C
63-66
1975
-
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-
1
-
2
1
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1
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1
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1
1
4
-
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1
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1
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2
1
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1
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1
1
4
-
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1
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641681
Van Hugo
Purification and properties of ...
Clostridium pasteurianum
Eur. J. Biochem.
48
455-463
1974
-
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2
12
3
-
5
7
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2
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1
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1
1
2
1
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1
1
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2
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12
-
3
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5
7
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1
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1
1
2
1
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1
1
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641682
Du Toit
-
Properties of 1-phosphofructok ...
Clostridium pasteurianum
Enzymologia
43
285-300
1972
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2
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1
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1
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1
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1
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2
-
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1
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1
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1
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-
640483
Sapico
D-fructose 1-phosphate kinase ...
Klebsiella aerogenes
J. Biol. Chem.
244
6280-6288
1969
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7
2
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1
1
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1
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1
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1
2
8
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1
1
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7
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2
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1
1
-
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-
1
-
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1
2
8
-
-
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1
1
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-
641677
Hanson
D-fructose 1-phosphate kinase, ...
Klebsiella aerogenes
J. Biol. Chem.
241
1644-1645
1966
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2
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1
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2
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1
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1
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2
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3
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1
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2
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1
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2
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1
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2
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3
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1
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641679
Reeves
1-Phosphofructokinase from an ...
Clostridium symbiosum
J. Biol. Chem.
241
1257-1261
1966
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2
1
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1
3
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1
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1
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1
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1
1
6
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1
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1
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2
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1
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1
3
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1
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1
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1
1
6
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1
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1
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