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Literature summary for 2.7.1.56 extracted from

  • Sapico, V.; Anderson, R.L.
    D-fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties (1969), J. Biol. Chem., 244, 6280-6288.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP additional Mg2+ reverses; at a Mg2+/ATP ratio below 2 Klebsiella aerogenes
citrate
-
Klebsiella aerogenes
D-fructose 1,6-bisphosphate
-
Klebsiella aerogenes
D-fructose 6-phosphate
-
Klebsiella aerogenes
GTP at a Mg2+/GTP ratio below 2 Klebsiella aerogenes
ITP at a Mg2+/ITP ratio below 2 Klebsiella aerogenes
additional information not inhibited by ATP; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by nucleoside monophosphates or diphosphates, D-mannose 6-phosphate, D-fructose, L-fructose 1-phosphate; not inhibited by phosphate, AMP Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
ATP
-
Klebsiella aerogenes
0.75
-
D-Fructose 1-phosphate
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
gel filtration Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
wild-type strain PRL-R3
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-20°C, 100 mM (NH4)2SO4, pH 7.5, at least 8 months Klebsiella aerogenes
-20°C, crude enzyme extract prepared in water, at least 3 weeks Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 1-phosphate no activity with D-fructose 6-phosphate Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 1-phosphate no activity with D-glucose 6-phosphate, D-glucose 1-phosphate Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 1-phosphate specific for D-fructose 1-phosphate, no activity with L-fructose 1-phosphate, D-fructose, D-mannose 6-phosphate Klebsiella aerogenes ADP + D-fructose 1,6-bisphosphate
-
?
CTP + D-fructose 1-phosphate poor substrate Klebsiella aerogenes CDP + D-fructose 1,6-bisphosphate
-
?
GTP + D-fructose 1-phosphate 35% of activity with ATP Klebsiella aerogenes GDP + D-fructose 1,6-bisphosphate
-
?
ITP + D-fructose 1-phosphate 43% of activity with ATP Klebsiella aerogenes IDP + D-fructose 1,6-bisphosphate
-
?
TTP + D-fructose 1-phosphate poor substrate Klebsiella aerogenes TDP + D-fructose 1,6-bisphosphate
-
?
UTP + D-fructose 1-phosphate poor substrate Klebsiella aerogenes UDP + D-fructose 1,6-bisphosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
7 8.7 approx. 80% of maximal activity at pH 7. approx. half-maximal activity at pH 8.7 Klebsiella aerogenes