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Literature summary for 2.7.1.52 extracted from

  • Wang, H.; Zhang, C.; Chen, H.; Yang, Q.; Zhou, X.; Gu, Z.; Zhang, H.; Chen, W.; Chen, Y.Q.
    Characterization of an fungal L-fucokinase involved in Mortierella alpina GDP-L-fucose salvage pathway (2016), Glycobiology, 26, 880-887.
    View publication on PubMed
Search for more literature for 2.7.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mortierella alpina

Inhibitors

Inhibitors Comment Organism Structure
Co2+ complete inhibition at 20 mM Mortierella alpina
EDTA
-
 Mortierella alpina
Fe2+ complete inhibition at 20 mM Mortierella alpina
GDP-L-fucose less than 30% inhibition at 0.2 mM Mortierella alpina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.59
-
 L-fucose at pH 7.4 and 37°C Mortierella alpina

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ best stimulation of activity in the presence of 2 mM Mg2+ Mortierella alpina
Mn2+ may replace Mg2+ Mortierella alpina
additional information not influenced by Ca2+, Cu2+, K+, Hg2+, Ni2+ and Zn2+ Mortierella alpina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, SDS-PAGE Mortierella alpina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-fucose Mortierella alpina
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
ATP + L-fucose Mortierella alpina ATCC 32222
-
 ADP + beta-L-fucose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mortierella alpina
-
-
-
Mortierella alpina ATCC 32222
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Mortierella alpina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-fucose
-
 Mortierella alpina ADP + beta-L-fucose 1-phosphate
-
 ?
ATP + L-fucose
-
 Mortierella alpina ATCC 32222 ADP + beta-L-fucose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Mortierella alpina

Synonyms

Synonyms Comment Organism
Fuk
-
 Mortierella alpina
L-fucokinase
-
 Mortierella alpina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Mortierella alpina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 more than 50% activity between 25 and 50°C Mortierella alpina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 37 the enzyme is stable for 2 h at 4 and 12°C. At 20°C, the enzyme shows 80% activity after 1 h and less than 20% activity after 2 h incubation. At 28°C, the enzyme is stable for 30 min, while at 37°C, the enzyme activity drops to about 37% after 30 min Mortierella alpina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.35
-
 L-fucose at pH 7.4 and 37°C Mortierella alpina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Mortierella alpina

pH Range

pH Minimum pH Maximum Comment Organism
6 10 more than 50% activity between pH 6.0 and 10.0 Mortierella alpina

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 12 After 30 min at pH 3,0, 5.0, 7.0, 8.0, 10.0, and 12.0, the enzyme shows about 25%, 50%, 90%, 100%, 75%, and 35% activity, respectively Mortierella alpina

Expression

Organism Comment Expression
Mortierella alpina the enzyme transcript level is up-regulated 4.3fold by nitrogen exhaustion when Mortierella alpina starts to accumulate lipid up