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Literature summary for 2.7.1.52 extracted from

  • Park, S.H.; Pastuszak, I.; Drake, R.; Elbein, A.D.
    Purification to apparent homogeneity and properties of pig kidney L-fucose kinase (1998), J. Biol. Chem., 273, 5685-5691.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is a valuable biochemical tool to prepare activated L-fucose derivatives for fucosylation reactions, enzyme is valuable for the formation of radiolabeled fucose 1-phosphate Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
D-arabinose 0.5 mM, 20% inhibition. 10 mM, 84% inhibition Sus scrofa
GDP-L-fucose
-
Sus scrofa
L-fucose 0.5 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
L-fucose pH 8.0, 37°C Sus scrofa
0.6
-
ATP pH 8.0, 37°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sus scrofa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, Co2+ stimulates with maximal activity at 3-5 mM, maximal activity is about 35-30% compared to activation by Mg2+ Sus scrofa
Fe2+ divalent cation required, Fe2+ stimulates nearly to the same degree as Mg2+, optimum activity at 10 mM Sus scrofa
Mg2+ absolute requirement for a divalent cation, best stimulation at 3 mM Sus scrofa
Mn2+ divalent cation required, Mn2+ stimulates with maximal activity at 3-5 mM, maximal activity is about 35-30% compared to activation by Mg2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
4 * 110000, SDS-PAGE Sus scrofa
440000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 6-deoxy-L-galactose Sus scrofa enzyme is part of a salvage pathway for reutilization of L-fucose ADP + 6-deoxy-L-galactose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
Sus scrofa
-
brain
-
Sus scrofa
-
heart
-
Sus scrofa
-
kidney
-
Sus scrofa
-
liver
-
Sus scrofa
-
lung
-
Sus scrofa
-
pancreas
-
Sus scrofa
-
spleen
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.273
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-deoxy-L-galactose
-
Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate beta-L-fucose 1-phosphate ?
ATP + 6-deoxy-L-galactose enzyme is part of a salvage pathway for reutilization of L-fucose Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
?
ATP + D-arabinose 9.3% of the activity with L-fucose Sus scrofa ?
-
?
ATP + D-glucose 2.5% of the activity with L-fucose Sus scrofa ?
-
?
ITP + 6-deoxy-L-galactose 2.5% of the activity with L-fucose Sus scrofa IDP + 6-deoxy-L-galactose 1-phosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 110000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
in Tris buffer about 80% of the activity in HEPES buffer Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
GDP-L-fucose
-
Sus scrofa