Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a valuable biochemical tool to prepare activated L-fucose derivatives for fucosylation reactions, enzyme is valuable for the formation of radiolabeled fucose 1-phosphate | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-arabinose | 0.5 mM, 20% inhibition. 10 mM, 84% inhibition | Sus scrofa | |
GDP-L-fucose | - |
Sus scrofa | |
L-fucose | 0.5 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
L-fucose | pH 8.0, 37°C | Sus scrofa | |
0.6 | - |
ATP | pH 8.0, 37°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, Co2+ stimulates with maximal activity at 3-5 mM, maximal activity is about 35-30% compared to activation by Mg2+ | Sus scrofa | |
Fe2+ | divalent cation required, Fe2+ stimulates nearly to the same degree as Mg2+, optimum activity at 10 mM | Sus scrofa | |
Mg2+ | absolute requirement for a divalent cation, best stimulation at 3 mM | Sus scrofa | |
Mn2+ | divalent cation required, Mn2+ stimulates with maximal activity at 3-5 mM, maximal activity is about 35-30% compared to activation by Mg2+ | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
4 * 110000, SDS-PAGE | Sus scrofa |
440000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-deoxy-L-galactose | Sus scrofa | enzyme is part of a salvage pathway for reutilization of L-fucose | ADP + 6-deoxy-L-galactose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aorta | - |
Sus scrofa | - |
brain | - |
Sus scrofa | - |
heart | - |
Sus scrofa | - |
kidney | - |
Sus scrofa | - |
liver | - |
Sus scrofa | - |
lung | - |
Sus scrofa | - |
pancreas | - |
Sus scrofa | - |
spleen | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.273 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-deoxy-L-galactose | - |
Sus scrofa | ADP + 6-deoxy-L-galactose 1-phosphate | beta-L-fucose 1-phosphate | ? | |
ATP + 6-deoxy-L-galactose | enzyme is part of a salvage pathway for reutilization of L-fucose | Sus scrofa | ADP + 6-deoxy-L-galactose 1-phosphate | - |
? | |
ATP + D-arabinose | 9.3% of the activity with L-fucose | Sus scrofa | ? | - |
? | |
ATP + D-glucose | 2.5% of the activity with L-fucose | Sus scrofa | ? | - |
? | |
ITP + 6-deoxy-L-galactose | 2.5% of the activity with L-fucose | Sus scrofa | IDP + 6-deoxy-L-galactose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 110000, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in Tris buffer about 80% of the activity in HEPES buffer | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
GDP-L-fucose | - |
Sus scrofa |