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Literature summary for 2.7.1.49 extracted from

  • Wrenger, C.; Eschbach, M.L.; Mueller, I.B.; Laun, N.P.; Begley, T.P.; Walter, R.D.
    Vitamin B1 de novo synthesis in the human malaria parasite Plasmodium falciparum depends on external provision of 4-amino-5-hydroxymethyl-2-methylpyrimidine (2006), Biol. Chem., 387, 41-51.
    View publication on PubMed
Search for more literature for 2.7.1.49

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BLR (DE3) Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 2-methyl-4-amino-5-hydroxymethylpyrimidine apparent value, in 100 mM triethanolamine or 50 mM potassium phosphate buffer, pH 7.5, 1 mM MgCl2, 500 mM ATP and 100 mM substrate Plasmodium falciparum
0.117
-
 ATP apparent value, in 100 mM triethanolamine or 50 mM potassium phosphate buffer, pH 7.5, 1 mM MgCl2, 500 mM ATP and 100 mM substrate Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 calculated from amino acid sequence Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.108
-
 using 2-methyl-4-amino-5-hydroxymethylpyrimidine as a substrate, at 37°C Plasmodium falciparum
0.169
-
 using bacimethrin as a substrate, at 37°C Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Plasmodium falciparum ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ir
ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine the enzyme shows lower affinity to 2-methyl-4-amino-5-hydroxymethylpyrimidine monophosphate than to 2-methyl-4-amino-5-hydroxymethylpyrimidine Plasmodium falciparum ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine
-
 ir
ATP + bacimethrin
-
 Plasmodium falciparum ADP + ?
-
 ?
additional information pyridoxine, pyridoxal, and pyridoxamine are no substrates Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, gel filtration Plasmodium falciparum

Synonyms

Synonyms Comment Organism
4-amino-5-hydroxymethyl-2-methylpyrimidine kinase
-
 Plasmodium falciparum
HMP kinase
-
 Plasmodium falciparum
HMP/HMP-P kinase
-
 Plasmodium falciparum
ThiD
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 2-methyl-4-amino-5-hydroxymethylpyrimidine apparent value, in 100 mM triethanolamine or 50 mM potassium phosphate buffer, pH 7.5, 1 mM MgCl2, 500 mM ATP and 100 mM substrate Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Plasmodium falciparum