BRENDA - Enzyme Database show
show all sequences of 2.7.1.49

Biosynthesis of hydroxymethylpyrimidine pyrophosphate in Saccharomyces cerevisiae

Kawasaki, Y.; Onozuka, M.; Mizote, T.; Nosaka, K.; Curr. Genet. 47, 156-162 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis
Saccharomyces cerevisiae
Engineering
Amino acid exchange
Commentary
Organism
additional information
phosphomethylpyrimidine kinase activity, EC 2.7.4.7, is abolished in C-terminally truncated isozymes Thi20p and Thi21p, the mutant yeast strains sow no thiamin phosphate synthesis activity
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
at 20 mM MgCl2
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
Saccharomyces cerevisiae
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
Saccharomyces cerevisiae YPH500
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
Saccharomyces cerevisiae
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
Saccharomyces cerevisiae YPH500
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21
-
Saccharomyces cerevisiae YPH500
-
2 isozymes Thi20p and Thi21p encoded by 2 redundant genes THI20 and THI21
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged isozymes Thi20p or Thi21p from Escherichia coli
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
thiamine synthesis from hydroxymethylpyrimidine activities in wild-type and mutant strains
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
661537
Saccharomyces cerevisiae
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
661537
Saccharomyces cerevisiae
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
-
?
additional information
the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p
661537
Saccharomyces cerevisiae
?
-
-
-
-
additional information
the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p
661537
Saccharomyces cerevisiae YPH500
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
phosphomethylpyrimidine kinase activity, EC 2.7.4.7, is abolished in C-terminally truncated isozymes Thi20p and Thi21p, the mutant yeast strains sow no thiamin phosphate synthesis activity
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
at 20 mM MgCl2
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
Saccharomyces cerevisiae
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
Saccharomyces cerevisiae YPH500
-
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
Saccharomyces cerevisiae
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
Saccharomyces cerevisiae YPH500
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged isozymes Thi20p or Thi21p from Escherichia coli
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
thiamine synthesis from hydroxymethylpyrimidine activities in wild-type and mutant strains
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
661537
Saccharomyces cerevisiae
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
661537
Saccharomyces cerevisiae
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
-
?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine
enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase
661537
Saccharomyces cerevisiae YPH500
ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
-
-
?
additional information
the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p
661537
Saccharomyces cerevisiae
?
-
-
-
-
additional information
the isozymes Thi20p and Thi21p are bifunctional and show both phosphomethylpyrimidine kinase, EC 2.7.4.7, and hydroxymethylpyrimidine kinase, EC 2.7.1.49, activity, Thi20p exhibits higher activity than Thi21p
661537
Saccharomyces cerevisiae YPH500
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 65000, recombinant His-tagged isozymes Thi20p or Thi21p, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 2.7.1.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738234
Castro-Fernandez
Emergence of pyridoxal phospho ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
FEBS Lett.
588
3068-3073
2014
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2
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1
1
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738451
Nodwell
A subfamily of bacterial ribok ...
Staphylococcus aureus, Staphylococcus aureus ATCC 700699
J. Am. Chem. Soc.
136
4992-4999
2014
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1
1
2
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1
6
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2
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4
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6
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722048
Nodwell
Rugulactone and its analogues ...
Staphylococcus aureus
ChemBioChem
13
1439-1446
2012
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3
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718477
French
Structure of trifunctional THI ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. D
67
784-791
2011
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1
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686824
Onozuka
Involvement of thiaminase II e ...
Saccharomyces cerevisiae
FEMS Yeast Res.
8
266-275
2008
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1
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1
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1
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1
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1
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2
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1
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1
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671903
Rapala-Kozik
Molecular characterization of ...
Zea mays
Biochem. J.
408
149-159
2007
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1
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7
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3
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1
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1
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7
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3
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1
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1
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1
1
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1
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672477
Wrenger
Vitamin B1 de novo synthesis i ...
Plasmodium falciparum
Biol. Chem.
387
41-51
2006
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1
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1
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674302
Karunakaran
Thiamine is synthesized by a s ...
Rhizobium leguminosarum
J. Bacteriol.
188
6661-6668
2006
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661316
Haas
Thi20, a remarkable enzyme fro ...
Saccharomyces cerevisiae
Bioorg. Chem.
33
338-344
2005
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661537
Kawasaki
Biosynthesis of hydroxymethylp ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae YPH500
Curr. Genet.
47
156-162
2005
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1
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1
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1
4
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662007
Park
Characterization of two kinase ...
Bacillus subtilis
J. Bacteriol.
186
1571-1573
2004
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641661
Mizote
Cloning and characterization o ...
Escherichia coli, Escherichia coli BL21(DE3) (pLysS)
Microbiology
145
495-501
1999
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639726
Kim
A Brassica cDNA clone encoding ...
Arabidopsis thaliana, Brassica napus
Plant Mol. Biol.
37
955-966
1998
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2
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641660
Reddick
-
Overexpression, purification a ...
Escherichia coli
Tetrahedron
54
15983-15991
1998
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1
1
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3
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1
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1
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1
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1
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641658
Mizote
Purification and properties of ...
Escherichia coli
Biochim. Biophys. Acta
991
109-113
1989
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1
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2
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2
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1
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1
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1
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2
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641657
Lewin
-
The biosynthesis of thiamine. ...
Saccharomyces cerevisiae
J. Biol. Chem.
236
2768-2771
1961
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1
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6
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1
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-
1
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-