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Literature summary for 2.7.1.48 extracted from
- Thymidine kinase and uridine-cytidine kinase from Entamoeba histolytica: cloning, characterization, and search for specific inhibitors (2009), Parasitology, 136, 595-602.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tag, expressed in Escherichia coli | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-propyl-2'-deoxyuridine | - |
Entamoeba histolytica |  |
| 6-(4-hexyloxyanilino)uracil | - |
Entamoeba histolytica | |
| cytidine | competitive inhibitor of phosphorylation of uridine | Entamoeba histolytica | |
| KCl | - |
Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
cytidine | - |
Entamoeba histolytica | |
| 0.74 | - |
uridine | - |
Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000 Da, SDS-PAGE | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | C4M4T4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion adsorption chromatography (Ni2+) | Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
- |
Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytidine + ATP | - |
Entamoeba histolytica | CMP + ADP | - |
? | |
| uridine + ATP | with GTP: 25% activity, with UTP or CTP: 0% activity | Entamoeba histolytica | UMP + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000 Da, SDS-PAGE | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| uridine-cytidine kinase | - |
Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Entamoeba histolytica |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.054 | - |
- |
Entamoeba histolytica | 6-(4-hexyloxyanilino)uracil | |
| 0.09 | - |
- |
Entamoeba histolytica | 5-propyl-2'-deoxyuridine | |
| 250 | - |
- |
Entamoeba histolytica | KCl | |
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