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Literature summary for 2.7.1.47 extracted from
- Purification, properties, and kinetics of D-ribulokinase from Aerobacter aerogenes (1979), J. Biol. Chem., 254, 3765-3771.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activation | Klebsiella aerogenes |  |
General Stability
| General Stability | Organism |
|---|---|
| D-ribulose stabilizes | Klebsiella aerogenes |
| EDTA or 2-mercaptoethanol stabilizes during purification | Klebsiella aerogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | product inhibition | Klebsiella aerogenes | |
| additional information | no inhibition by D-ribulose 5-phosphate | Klebsiella aerogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study at 28°C | Klebsiella aerogenes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
2 * 59000, SDS-PAGE | Klebsiella aerogenes |
| 116000 | - |
gel filtration | Klebsiella aerogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribulose | Klebsiella aerogenes | inducible enzyme of ribitol catabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
mutant, constitutive for D-ribulokinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-ribulose = ADP + D-ribulose 5-phosphate | random bi bi reaction mechanism | Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 152 | - |
- |
Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, about 10% loss of activity within 6 months | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribulose | - |
Klebsiella aerogenes | ADP + D-ribulose 5-phosphate | - |
? | |
| ATP + D-ribulose | inducible enzyme of ribitol catabolism | Klebsiella aerogenes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 59000, SDS-PAGE | Klebsiella aerogenes |
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