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Literature summary for 2.7.1.45 extracted from
- Identification and characterization of Thermoplasma acidophilum 2-keto-3-deoxy-D-gluconate kinase: A new class of sugar kinases (2005), Biotechnol. Bioprocess Eng., 10, 535-539.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| dephosphorylation with potato acid phosphatase or bacterial alkaline phosphatase leads to complete loss of activity | Thermoplasma acidophilum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
8 * 34000, denaturing SDS-PAGE | Thermoplasma acidophilum |
| 36390 | - |
calculated from amino acid sequence | Thermoplasma acidophilum |
| 260000 | - |
gel filtration | Thermoplasma acidophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | dephosphorylation with potato acid phosphatase or bacterial alkaline phosphatase, leads to complete loss of activity | Thermoplasma acidophilum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sepharose column chromatography, Q Sepharose column chromatography, Affi-gel blue column chromatography, Sephadex G-75 gel filtration, and Mono Q HR 5/5 column chromatography | Thermoplasma acidophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
15.6 units/mg, at 50°C in 50 mM Tris-HCl, pH 8.0 | Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Thermoplasma acidophilum | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? |  |
| additional information | D-gluconate, 2-keto-D-gluconate, 5-keto-D-gluconate, N-acetylglucosamine, and D-xylose are no substrates | Thermoplasma acidophilum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homooctamer | 8 * 34000, denaturing SDS-PAGE | Thermoplasma acidophilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-keto-3-deoxy-D-gluconate kinase | - |
Thermoplasma acidophilum |
| KDG kinase | - |
Thermoplasma acidophilum |
| Ta0122 | - |
Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermoplasma acidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Thermoplasma acidophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Thermoplasma acidophilum | |
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Release 2023.1 (January 2023)
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