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Literature summary for 2.7.1.45 extracted from

  • Inagaki, E.; Ukita, Y.; Kumei, M.; Kajihara, Y.; Tahirov, T.H.
    Crystallization and preliminary crystallographic analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus (2004), Acta Crystallogr. Sect. D, 60, 761-763.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, free enzyme or in complex with ATP and 2-dehydro-3-deoxy-D-gluconate, method 1: automated microbatch method, 500 nl 23 mg/ml protein mixed with 500 nl screen solution containing 44% v/v methylpentanediol, 10% v/v dioxane, 0.1 M Na HEPES, pH 7.7, and covered with 0.015 ml silicone and parraffin oil mixture, 18°C, crystals grow to final dimensions within 1 month after appearance, method 2: sitting drop vapour diffusion method at 25°C, 0.001 ml 10 mg/ml protein solution mixed with equal volume of reservoir solution and equilibrated against 0.1 ml reservoir solution containing 28% v/v methylpentanediol, 10 mM CaCl2, 0.1 M trisodium citrate buffer, pH 5.6, or for cocrystallization of enzyme with ligands containing 0.35-0.45 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.1-3.2 A resolution Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-dehydro-3-deoxy-D-gluconate Thermus thermophilus
-
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-dehydro-3-deoxy-D-gluconate
-
Thermus thermophilus ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
?

Synonyms

Synonyms Comment Organism
2-keto-3-deoxygluconate kinase
-
Thermus thermophilus
KDGK
-
Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Thermus thermophilus