Cloned (Comment) | Organism |
---|---|
His-tag, expressed in Escherichia coli BL21(de3)plysS | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
A386S | possibly critical role in catalysis, relative activity: 90% (wild-type: 100%) | Arabidopsis thaliana |
A41E | possibly critical for sugar binding, relative activity: 0% (wild-type: 100%) | Arabidopsis thaliana |
Y250F | possibly important for substrate specificity (galacturonic acid /glucuronic acid), relative activity: 90% (wild-type: 100%), 50% activity with D-glucuronic acid (wild-type: 0%) | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | addition (4 mM) results in 40% reduction of activity | Arabidopsis thaliana | |
EDTA | - |
Arabidopsis thaliana | |
additional information | no inhibition is observed by CTP, GTP ITP,TTP, or UTP (4 mM) | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0708 | - |
D-galacturonate | wild-type protein | Arabidopsis thaliana | |
0.195 | - |
ATP | wild-type protein | Arabidopsis thaliana | |
0.395 | - |
D-galacturonate | Y250F mutant protein | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mn2+ (32% relative activity) and Ca2+ (61% relative activity) can substitute for Mg2+ | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VYG2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Storage Stability | Organism |
---|---|
-20°C, crude recombinant enzyme is stable | Arabidopsis thaliana |
-20°C, purified enzyme preparations lose more than 90% of activity within one week | Arabidopsis thaliana |
-80°C, stable for several months when diluted with glycerol to 25% (v/v) and flash-frozen in liquid nitrogen | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + ATP | wild-type protein: no activity with D-galactose, D-glucose, D-fructose, D-mannose, D-gluconic acid, D-xylose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-glucosamine, D-arabinose, L-arabinose, L-rhamnose,or L-Fucose, these monosaccharides (4 mM) have no effect on activity in the presence of galacturonic acid, neither CTP, GTP ITP,TTP, nor UTP are substrates | Arabidopsis thaliana | 1-phospho-alpha-D-galacturonate + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-D-galacturonic acid kinase | - |
Arabidopsis thaliana |
alpha-D-galacturonic acid-1-phosphate kinase | - |
Arabidopsis thaliana |
GalAK | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 42 | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
no activity at 55°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.8 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.3 | 9.5 | - |
Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
Alpha-D-glucuronic acid | Y250F mutant protein | Arabidopsis thaliana | |
6.3 | - |
ATP | wild-type protein | Arabidopsis thaliana | |
24 | - |
D-galacturonate | Y250F mutant protein | Arabidopsis thaliana | |
36 | - |
D-galacturonate | wild-type protein | Arabidopsis thaliana |