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Literature summary for 2.7.1.44 extracted from

  • Yang, T.; Bar-Peled, L.; Gebhart, L.; Lee, S.G.; Bar-Peled, M.
    Identification of galacturonic acid-1-phosphate kinase, a new member of the GHMP kinase superfamily in plants, and comparison with galactose-1-phosphate kinase (2009), J. Biol. Chem., 284, 21526-21535.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tag, expressed in Escherichia coli BL21(de3)plysS Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A386S possibly critical role in catalysis, relative activity: 90% (wild-type: 100%) Arabidopsis thaliana
A41E possibly critical for sugar binding, relative activity: 0% (wild-type: 100%) Arabidopsis thaliana
Y250F possibly important for substrate specificity (galacturonic acid /glucuronic acid), relative activity: 90% (wild-type: 100%), 50% activity with D-glucuronic acid (wild-type: 0%) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
ADP addition (4 mM) results in 40% reduction of activity Arabidopsis thaliana
EDTA
-
Arabidopsis thaliana
additional information no inhibition is observed by CTP, GTP ITP,TTP, or UTP (4 mM) Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0708
-
D-galacturonate wild-type protein Arabidopsis thaliana
0.195
-
ATP wild-type protein Arabidopsis thaliana
0.395
-
D-galacturonate Y250F mutant protein Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ (32% relative activity) and Ca2+ (61% relative activity) can substitute for Mg2+ Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8VYG2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Storage Stability

Storage Stability Organism
-20°C, crude recombinant enzyme is stable Arabidopsis thaliana
-20°C, purified enzyme preparations lose more than 90% of activity within one week Arabidopsis thaliana
-80°C, stable for several months when diluted with glycerol to 25% (v/v) and flash-frozen in liquid nitrogen Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + ATP wild-type protein: no activity with D-galactose, D-glucose, D-fructose, D-mannose, D-gluconic acid, D-xylose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-glucosamine, D-arabinose, L-arabinose, L-rhamnose,or L-Fucose, these monosaccharides (4 mM) have no effect on activity in the presence of galacturonic acid, neither CTP, GTP ITP,TTP, nor UTP are substrates Arabidopsis thaliana 1-phospho-alpha-D-galacturonate + ADP
-
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Synonyms

Synonyms Comment Organism
alpha-D-galacturonic acid kinase
-
Arabidopsis thaliana
alpha-D-galacturonic acid-1-phosphate kinase
-
Arabidopsis thaliana
GalAK
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 42
-
Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
no activity at 55°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
3.3 9.5
-
Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.76
-
Alpha-D-glucuronic acid Y250F mutant protein Arabidopsis thaliana
6.3
-
ATP wild-type protein Arabidopsis thaliana
24
-
D-galacturonate Y250F mutant protein Arabidopsis thaliana
36
-
D-galacturonate wild-type protein Arabidopsis thaliana