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Literature summary for 2.7.1.41 extracted from

  • Mesak, L.R.; Dahl, M.K.
    Purification and enzymatic characterization of PgcM: A beta-phosphoglucomutase and glucose-1-phosphate phosphodismutase of Bacillus subtilis (2000), Arch. Microbiol., 174, 256-264.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
pgcM gene, encoding PgcM, a 226 amino acids protein with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, is cloned, sequenced and used to construct a plasmid-based overexpression system for PcgM in Bacillus megaterium DSM 319 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
beta-D-glucose 1-phosphate
-
Bacillus subtilis
0.021
-
alpha-D-glucose 1-phosphate
-
Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities Bacillus subtilis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 as cofactor, optimal concentration: 10-15 mM Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
3 * 26500, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, SDS-PAGE Bacillus subtilis
28000
-
3 * 28000, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, estimated from the amino acid sequence data Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis carbohydrate metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
wild-type strain 168
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PgcM expressed in Bacillus megaterium DSM 319 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate
-
Bacillus subtilis alpha-D-glucose + alpha-D-glucose 1,6-bisphosphate
-
?
beta-D-glucose 1-phosphate + beta-D-glucose 1-phosphate
-
Bacillus subtilis beta-D-glucose + beta-D-glucose 1,6-bisphosphate
-
?
D-glucose 1-phosphate + D-glucose 1-phosphate PgcM uses the alpha- and beta-forms of glucose 1-phosphate as substrates, higher affinity for the beta-form, binding of substrates and cofactors trigger a conformational change of PgcM Bacillus subtilis D-glucose + D-glucose 1,6-bisphosphate
-
?
additional information bifunctional PgcM with beta-phosphoglucomutase, EC 5.4.2.1, and glucose 1-phosphate phosphodismutase activities Bacillus subtilis ?
-
?
additional information carbohydrate metabolism Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
trimer 3 * 28000, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, estimated from the amino acid sequence data Bacillus subtilis
trimer 3 * 26500, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
PgcM bifunctional PgcM with beta-phosphoglucomutase, EC 5.4.2.1, and glucose 1-phosphate phosphodismutase activities Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
30 min, stable, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities Bacillus subtilis