Cloned (Comment) | Organism |
---|---|
pgcM gene, encoding PgcM, a 226 amino acids protein with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, is cloned, sequenced and used to construct a plasmid-based overexpression system for PcgM in Bacillus megaterium DSM 319 | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
beta-D-glucose 1-phosphate | - |
Bacillus subtilis | |
0.021 | - |
alpha-D-glucose 1-phosphate | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | recombinant PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities | Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2 as cofactor, optimal concentration: 10-15 mM | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26500 | - |
3 * 26500, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, SDS-PAGE | Bacillus subtilis |
28000 | - |
3 * 28000, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, estimated from the amino acid sequence data | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus subtilis | carbohydrate metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
wild-type strain 168 | - |
Purification (Comment) | Organism |
---|---|
recombinant PgcM expressed in Bacillus megaterium DSM 319 | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate | - |
Bacillus subtilis | alpha-D-glucose + alpha-D-glucose 1,6-bisphosphate | - |
? | |
beta-D-glucose 1-phosphate + beta-D-glucose 1-phosphate | - |
Bacillus subtilis | beta-D-glucose + beta-D-glucose 1,6-bisphosphate | - |
? | |
D-glucose 1-phosphate + D-glucose 1-phosphate | PgcM uses the alpha- and beta-forms of glucose 1-phosphate as substrates, higher affinity for the beta-form, binding of substrates and cofactors trigger a conformational change of PgcM | Bacillus subtilis | D-glucose + D-glucose 1,6-bisphosphate | - |
? | |
additional information | bifunctional PgcM with beta-phosphoglucomutase, EC 5.4.2.1, and glucose 1-phosphate phosphodismutase activities | Bacillus subtilis | ? | - |
? | |
additional information | carbohydrate metabolism | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 28000, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, estimated from the amino acid sequence data | Bacillus subtilis |
trimer | 3 * 26500, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
PgcM | bifunctional PgcM with beta-phosphoglucomutase, EC 5.4.2.1, and glucose 1-phosphate phosphodismutase activities | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
30 min, stable, PgcM with beta-phosphoglucomutase and glucose 1-phosphate phosphodismutase activities | Bacillus subtilis |