Literature summary for 2.7.1.40 extracted from

Mattice, A.M.S.; MacLean, I.A.; Childers, C.L.; Storey, K.B.
Characterization of pyruvate kinase from the anoxia tolerant turtle, Trachemys scripta elegans a potential role for enzyme methylation during metabolic rate depression (2018), PeerJ, 6, e4918 .

Activating Compound

Activating Compound	Comment	Organism
fructose 1,6-bisphosphate	Ka of liver enzyme is 0.029 mM, of anoxic liver enzyme 0.0032 mM	Trachemys scripta elegans
additional information	L-aspartate has no significant effect on the activity of the liver enzyme at 0-20 mM	Trachemys scripta elegans
additional information	neither L-aspartate nor fructose 1,6-bisphosphate has an influence on muscle pyruvate kinase in terms of activation	Trachemys scripta elegans

Inhibitors	Comment	Organism
ATP	-	Trachemys scripta elegans
KCl	-	Trachemys scripta elegans
L-alanine	-	Trachemys scripta elegans
Lactate	-	Trachemys scripta elegans
additional information	no inhibiton by L-alanine	Trachemys scripta elegans
Urea	-	Trachemys scripta elegans

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.011	-	phosphoenolpyruvate	muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans
0.029	-	phosphoenolpyruvate	muscle enzyme, pH 6.6, 22°C	Trachemys scripta elegans
0.051	-	phosphoenolpyruvate	liver enzyme, pH 6.6, 22°C	Trachemys scripta elegans
0.057	-	phosphoenolpyruvate	anoxic muscle enzyme, pH 6.6, 22°C	Trachemys scripta elegans
0.061	-	phosphoenolpyruvate	anoxic muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans
0.092	-	phosphoenolpyruvate	anoxic liver enzyme, pH 6.6, 22°C	Trachemys scripta elegans
0.12	-	ADP	liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans
0.13	-	ADP	anoxic liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans
0.2	-	ADP	muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans
0.28	-	ADP	anoxic muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans
0.31	-	phosphoenolpyruvate	liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans
0.55	-	phosphoenolpyruvate	anoxic liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Trachemys scripta elegans

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + phosphoenolpyruvate	Trachemys scripta elegans	-	ATP + pyruvate	-	?

Organism	UniProt	Comment	Textmining
Trachemys scripta elegans	Q9I8C5	-	-
Trachemys scripta elegans	Q9PU23	-	-

Posttranslational Modification	Comment	Organism
alkylation	lysine methylation increases dramatically in the anoxic state by 70% relative to the control condition	Trachemys scripta elegans
alkylation	lysine methylation of muscle PK increases significantly by 43% in the anoxic state compared to control	Trachemys scripta elegans
phosphoprotein	anoxic red muscle PK shows a 42% increase in threonine phosphorylation relative to the control. But there are no significant differences in neither tyrosine nor serine residue phosphorylation between the control and anoxic states	Trachemys scripta elegans
phosphoprotein	liver PK shows 60% increased threonine phosphorylation levels in the anoxic state compared to control. Conversely, phosphorylation on tyrosine residues decreases by 26% for anoxic PK relative to control, whereas serine phosphorylation is unchanged	Trachemys scripta elegans

Purification (Comment)	Organism
native enzyme from liver by two different steps of cation exchange chromatography, 7.3fold	Trachemys scripta elegans
native enzyme from red muscle by cation exchange chromatography, 82.8-97.6fold to homogeneity	Trachemys scripta elegans

Source Tissue	Comment	Organism	Textmining
liver	liver-specific isozyme	Trachemys scripta elegans	-
muscle	muscle-specific isozyme	Trachemys scripta elegans	-

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.49	1.32	purified native liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans
11.8	14.5	purified native muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphoenolpyruvate	-	Trachemys scripta elegans	ATP + pyruvate	-	?

Subunits	Comment	Organism
?	x * 57000, SDS-PAGE	Trachemys scripta elegans

Synonyms	Comment	Organism
liver PK	-	Trachemys scripta elegans
muscle PK	-	Trachemys scripta elegans

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Trachemys scripta elegans

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Trachemys scripta elegans

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.01	-	anoxic liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	L-alanine
0.1	-	liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	L-alanine
1.1	-	anoxic liver enzyme, pH 7.2, 22°C, in presence of fructose 1,6-bisphosphate	Trachemys scripta elegans	ATP
2	-	liver enzyme, pH 7.2, 22°C, in presence of fructose 1,6-bisphosphate	Trachemys scripta elegans	ATP
2.5	-	liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	ATP
2.7	-	anoxic liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	ATP
7.9	-	anoxic muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	ATP
11.6	-	muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	ATP
34.4	-	muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Lactate
51	-	anoxic muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	KCl
54	-	muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	KCl
131	-	anoxic liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Lactate
177.6	-	anoxic muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Lactate
187	-	liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Lactate
410	-	anoxic liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	KCl
433	-	liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	KCl
1406	-	anoxic muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Urea
1410	-	liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Urea
1901	-	muscle enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Urea
1980	-	anoxic liver enzyme, pH 7.2, 22°C	Trachemys scripta elegans	Urea