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Literature summary for 2.7.1.40 extracted from
- Zinc inhibition of pyruvate kinase of M-type isozyme (2017), Biometals, 30, 335-340 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | inhibits the M-(muscle)-type isozyme of pyruvate kinase. Zn2+ inhibits pyruvate kinase uncompetitively with respect to the substrate phosphoenolpyruvate (PEP), and competitively with respect to ADP. Zn2+ as a ZnADP complex acts as competitive and uncompetitive inhibitors of the enzyme with respect to the substrate ADP and PEP, respectively. Zn2+ forms a ZnADP complex, which may bind to the ADP-binding site of the free enzyme with the Ki value of 1.4 microM causing competitive inhibition, or to the ADP-site of the enzyme-PEP complex with 2.6 microM resulting in uncompetitive inhibition | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | Homo sapiens | - |
ATP + pyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P14618 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | M (muscle)-type isozyme | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Homo sapiens | ATP + pyruvate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
| 0.0014 | - |
Zn2+ | Zn2+ bound to the ADP-binding site of the free enzyme, pH 7.4, 37°C | Homo sapiens | |
| 0.0026 | - |
Zn2+ | Zn2+ bound to the ADP-site of the enzyme-PEP complex, pH 7.4, 37°C | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the inhibition of pyruvate kinase by Zn2+ may be responsible for the cytotoxicity of this metal by decreasing glycolytic flux | Homo sapiens |
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