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Literature summary for 2.7.1.40 extracted from

  • Boehme, C.; Bieber, F.; Linnemann, J.; Breitling, R.; Lorkowski, S.; Reissmann, S.
    Chemical and enzymatic characterization of recombinant rabbit muscle pyruvate kinase (2013), Biol. Chem., 394, 695-701.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Oryctolagus cuniculus
Cu2+
-
Oryctolagus cuniculus
Mn2+
-
Oryctolagus cuniculus
Ni2+
-
Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
phosphoenolpyruvate recombinant enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus
0.076
-
phosphoenolpyruvate native enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus
0.357
-
ADP native enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus
0.365
-
ADP recombinant enzyme, at pH 7.4 and 37°C Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ necessary for full enzymatic activity Oryctolagus cuniculus
Mg2+ necessary for full enzymatic activity Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
recombinant enzyme, gel filtration Oryctolagus cuniculus
59000
-
native enzyme, gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphoenolpyruvate Oryctolagus cuniculus
-
ATP + pyruvate
-
?
TDP + phosphoenolpyruvate Oryctolagus cuniculus
-
TTP + pyruvate 95% yield ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IDA affinity column chromatography, and gel filtration Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Oryctolagus cuniculus
-

Storage Stability

Storage Stability Organism
-20°C, with 50% (v/v) glycerol, 72 h, no loss of activity Oryctolagus cuniculus
30°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 70% loss of activity Oryctolagus cuniculus
37°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 95% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Oryctolagus cuniculus ATP + pyruvate
-
?
additional information the recombinant enzyme shows the following range in its substrate specificity: ADP>dADP>dGDP>dCDP>TDP Oryctolagus cuniculus ?
-
?
TDP + phosphoenolpyruvate
-
Oryctolagus cuniculus TTP + pyruvate 95% yield ?

Subunits

Subunits Comment Organism
homodimer 2 * 29495, MALDI-TOF spectrometry Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
M1-PK
-
Oryctolagus cuniculus
pyruvate phosphotransferase
-
Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.8
-
Oryctolagus cuniculus