Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Oryctolagus cuniculus | |
Cu2+ | - |
Oryctolagus cuniculus | |
Mn2+ | - |
Oryctolagus cuniculus | |
Ni2+ | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
phosphoenolpyruvate | recombinant enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus | |
0.076 | - |
phosphoenolpyruvate | native enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus | |
0.357 | - |
ADP | native enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus | |
0.365 | - |
ADP | recombinant enzyme, at pH 7.4 and 37°C | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | necessary for full enzymatic activity | Oryctolagus cuniculus | |
Mg2+ | necessary for full enzymatic activity | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
recombinant enzyme, gel filtration | Oryctolagus cuniculus |
59000 | - |
native enzyme, gel filtration | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | Oryctolagus cuniculus | - |
ATP + pyruvate | - |
? | |
TDP + phosphoenolpyruvate | Oryctolagus cuniculus | - |
TTP + pyruvate | 95% yield | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-IDA affinity column chromatography, and gel filtration | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Storage Stability | Organism |
---|---|
-20°C, with 50% (v/v) glycerol, 72 h, no loss of activity | Oryctolagus cuniculus |
30°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 70% loss of activity | Oryctolagus cuniculus |
37°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 95% loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Oryctolagus cuniculus | ATP + pyruvate | - |
? | |
additional information | the recombinant enzyme shows the following range in its substrate specificity: ADP>dADP>dGDP>dCDP>TDP | Oryctolagus cuniculus | ? | - |
? | |
TDP + phosphoenolpyruvate | - |
Oryctolagus cuniculus | TTP + pyruvate | 95% yield | ? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 29495, MALDI-TOF spectrometry | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
M1-PK | - |
Oryctolagus cuniculus |
pyruvate phosphotransferase | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.8 | - |
Oryctolagus cuniculus |