Literature summary for 2.7.1.40 extracted from

Deng, Y.; Olson, D.G.; Zhou, J.; Herring, C.D.; Joe Shaw, A.; Lynd, L.R.
Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum (2013), Metab. Eng., 15, 151-158.

Search for more literature for 2.7.1.40

Cloned(Commentary)

Cloned (Comment)	Organism
enzyme overexpression in Clostridium thermocellum strain dsm1313 resulting in strains DS8, YD01, and YD02	Thermoanaerobacterium saccharolyticum

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant overexpression of the enzyme in Clostridium thermocellum improves carbon flux to ethanol in the transformed cells. Clostridium thermocellum strain YD01 with exogenous pyruvate kinase, in which phosphoenolpyruvate carboxykinase expression is diminished by modifying the start codon from ATG to GTG, exhibits 3.25fold higher ethanol yield than the wild-type Clostridium thermocellum strain. A second strain, YD02 with exogenous pyruvate kinase, in which the gene for malic enzyme and part of malate dehydrogenase are deleted, has over 3fold higher ethanol yield than the wild-type strain	Thermoanaerobacterium saccharolyticum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thermoanaerobacterium saccharolyticum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + pyruvate	Thermoanaerobacterium saccharolyticum	-	ADP + phosphoenolpyruvate	-	r

Organism

Organism	UniProt	Comment	Textmining
no activity in Clostridium thermocellum	-	-	-
Thermoanaerobacterium saccharolyticum	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.001	-	below, wild-type strain DSM133, pH 7.5, 55°C	Thermoanaerobacterium saccharolyticum
0.14	-	mutant strain YD01, pH 7.5, 55°C	Thermoanaerobacterium saccharolyticum
0.52	-	mutant strain DS8, pH 7.5, 55°C	Thermoanaerobacterium saccharolyticum
0.84	-	mutant strain YD02, pH 7.5, 55°C	Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pyruvate	-	Thermoanaerobacterium saccharolyticum	ADP + phosphoenolpyruvate	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	assay at	Thermoanaerobacterium saccharolyticum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Thermoanaerobacterium saccharolyticum

Cofactor

Cofactor	Comment	Organism	Structure
ADP	-	Thermoanaerobacterium saccharolyticum
ATP	-	Thermoanaerobacterium saccharolyticum

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Release 2023.1 (January 2023)