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Literature summary for 2.7.1.40 extracted from

  • Diaz-Jullien, C.; Moreira, D.; Sarandeses, C.S.; Covelo, G.; Barbeito, P.; Freire, M.
    The M2-type isoenzyme of pyruvate kinase phosphorylates prothymosin alpha in proliferating lymphocytes (2011), Biochim. Biophys. Acta, 1814, 355-365.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-fructose 1,6-bisphosphate phosphorylation of serine and threonine residues is, besides being essential for isozyme M2 catalytic activity, induces a trimeric association of the ProTalpha kinase. This association can be shifted to a tetrameric form by fructose 1,6-bisphosphate, which results in a decrease in ProTalphaK activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + prothymosin alpha Homo sapiens
-
ADP + phospho-prothymosin alpha
-
?
ATP + pyruvate Homo sapiens
-
ADP + phosphoenolpyruvate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
M2 isoform
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of serine and threonine residues is, besides being essential for isozyme M2 catalytic activity, induces a trimeric association of the ProTalpha kinase. This association can be shifted to a tetrameric form by fructose 1,6-bisphosphate, which results in a decrease in ProTalphaK activity Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
native enzyme from cytosol by ammonium sulfate fractionation and prothymosin alpha affinity and ion exchange chromatography, and chromatography on a phosphocellulose resin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
B-lymphocyte M2-type isoenzyme Homo sapiens
-
NC-37 cell a lymphoblastoid cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + prothymosin alpha
-
Homo sapiens ADP + phospho-prothymosin alpha
-
?
ATP + prothymosin alpha isozyme M2-type acts as a ProTalpha kinase phosphorylating ProTalpha Homo sapiens ADP + phospho-prothymosin alpha
-
?
ATP + pyruvate
-
Homo sapiens ADP + phosphoenolpyruvate
-
?

Synonyms

Synonyms Comment Organism
ProTalphaK
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
physiological function acquisition of ProTalpha kinase activity by M2 isozyme seems to be due to the phosphorylation of serine and threonine residues, which, besides being essential for its catalytic activity, induces a trimeric association of ProTalpha kinase. Cytosolic phosphorylation of ProTaalpha, which then migrates to the nucleus, where it influences chromatin activity Homo sapiens