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Literature summary for 2.7.1.40 extracted from
- Purification and kinetic properties of pyruvate kinase isoenzymes of Salmonella typhimurium (1987), Biochem. J., 241, 573-581.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | activation | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| D-fructose 1,6-diphosphate | kinetics | Salmonella enterica subsp. enterica serovar Typhimurium | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | 0.17 | ADP | isozyme II, 30°C, pH 6.8 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.2 | 0.35 | ADP | enzyme form I | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.2 | 0.35 | ADP | 30°C, pH 6.8 | Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | positive cooperativity | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
4 * 47000, isozyme II, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 54000 | - |
4 * 54000, isozyme I, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 190000 | - |
isozyme PKII, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
| 220000 | - |
isozyme PKII, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
strain LT-2, highest activity in cells grown on glycolytic substrates, e.g. glucose, galactose, fructose or glycerol | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoforms, I and II | Salmonella enterica subsp. enterica serovar Typhimurium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in 50 mM Tris-HCl buffer, isozyme I up to 10 days, isozyme II 4 days | Salmonella enterica subsp. enterica serovar Typhimurium |
| EDTA, 2-mercaptoethanol, dithiothreitol, glycerol and KCl stabilize, being more effective at refrigerated conditions than at room temperature | Salmonella enterica subsp. enterica serovar Typhimurium |
| frozen, in 50 mM Tris-HCl buffer, isozyme I at least 9 months, isozyme II 4 months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Salmonella enterica subsp. enterica serovar Typhimurium | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 54000, isozyme I, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| tetramer | 4 * 47000, isozyme II, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
at pH 7-8, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
| 37 | - |
at pH 7-8, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
| 55 | - |
15 min, 12% loss of activity, isozyme I, inactivation of isozyme II | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
more active in HEPES than in other buffers | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.8 | increase of activity in this range, decline at higher pH-values | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | at 25°C and 37°C, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| AMP | activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
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