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Literature summary for 2.7.1.40 extracted from

  • Duncan, D.; McGowan, J.; Price, N.C.
    Properties of pyruvate kinase from Schizosaccharomyces pombe (1989), Biochem. Soc. Trans., 17, 560-561.
No PubMed abstract available
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-diphosphate activation Schizosaccharomyces pombe
D-fructose 1,6-diphosphate more evident in the presence of glycerol Schizosaccharomyces pombe

General Stability

General Stability Organism
dialysis against 10 mM sodium phosphate or Tris-HCl, pH 7.5, for 18 h at 4°C, inactivates Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Schizosaccharomyces pombe
100000
-
 gel filtration Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
 fission yeast
-

Purification (Commentary)

Purification (Comment) Organism
partial, dye-ligand chromatography Schizosaccharomyces pombe

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Schizosaccharomyces pombe
-

Storage Stability

Storage Stability Organism
-20°C, in 10 mM Tris-HCl or sodium phosphate buffer, pH 7.5, 50% v/v glycerol, 18 h Schizosaccharomyces pombe
4°C, in 10 mM Tris-HCl or sodium phosphate buffer, pH 7.5, 50% v/v glycerol, 18 h Schizosaccharomyces pombe
glycerol stabilizes during storage Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
 Schizosaccharomyces pombe ATP + pyruvate
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Schizosaccharomyces pombe