Any feedback?
Literature summary for 2.7.1.40 extracted from
- Pyruvate kinase isozymes from the green alga, Selenastrum minutum. II. Kinetic and regulatory properties (1989), Arch. Biochem. Biophys., 269, 228-238.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| asparagine | activation | Monoraphidium minutum |  |
| asparagine | kinetics | Monoraphidium minutum | |
| D-glucose 1-phosphate | slight activation, not isozyme PKp | Monoraphidium minutum | |
| D-glucose 6-phosphate | activation | Monoraphidium minutum | |
| D-glucose 6-phosphate | not: isozyme PKp | Monoraphidium minutum | |
| dihydroxyacetone phosphate | activation | Monoraphidium minutum | |
| dihydroxyacetone phosphate | kinetics | Monoraphidium minutum | |
| isoleucine | slight activation, kinetics | Monoraphidium minutum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | - |
Monoraphidium minutum | |
| 2-phosphoglycerate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| 3-phosphoglycerate | - |
Monoraphidium minutum | |
| aspartate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| ATP | MgATP2- | Monoraphidium minutum | |
| citrate | - |
Monoraphidium minutum | |
| D-ribose 5-phosphate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| D-ribulose 1,5-bisphosphate | isozyme PKp, not PKc | Monoraphidium minutum | |
| fructose 1,6-diphosphate | - |
Monoraphidium minutum | |
| glucose 6-phosphate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| isocitrate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| malate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| MgATP2- | - |
Monoraphidium minutum | |
| oxalate | - |
Monoraphidium minutum | |
| phosphate | - |
Monoraphidium minutum | |
| Phosphoglycolate | only isozyme PKp, not PKc | Monoraphidium minutum | |
| tyrosine | - |
Monoraphidium minutum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | 0.05 | ADP | PKc-isozyme | Monoraphidium minutum | |
| 0.049 | 0.1 | IDP | cosubstrate phosphoenolpyruvate | Monoraphidium minutum | |
| 0.049 | 0.1 | IDP | isozyme PKc | Monoraphidium minutum | |
| 0.18 | - |
phosphoenolpyruvate | isozyme PKp | Monoraphidium minutum | |
| 0.2 | 0.35 | ADP | PKp-isozyme | Monoraphidium minutum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | 2 isozymes: a cytosolic and a chloroplastid one | Monoraphidium minutum | 9507 | - |
| cytosol | 2 isozymes: a cytosolic and a plastid one | Monoraphidium minutum | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cation | requirement | Monoraphidium minutum | |
| divalent cation | requires both a divalent and a monovalent cation | Monoraphidium minutum | |
| K+ | - |
Monoraphidium minutum | |
| Mg2+ | - |
Monoraphidium minutum | |
| Mn2+ | can partially replace Mg2+ | Monoraphidium minutum | |
| Mn2+ | requirement | Monoraphidium minutum | |
| monovalent cation | requirement | Monoraphidium minutum | |
| monovalent cation | requires both a divalent and a monovalent cation | Monoraphidium minutum | |
| NH4+ | requirement | Monoraphidium minutum | |
| NH4+ | can replace K+ | Monoraphidium minutum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monoraphidium minutum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Monoraphidium minutum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Monoraphidium minutum | ATP + pyruvate | - |
? | |
| CDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | CTP + pyruvate | - |
? | |
| CDP + phosphoenolpyruvate | reaction with PKp-isozyme at 30%, with PKc-isozyme at 12% the rate of ADP | Monoraphidium minutum | CTP + pyruvate | - |
? | |
| GDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | GTP + pyruvate | - |
? | |
| GDP + phosphoenolpyruvate | reaction for PKc-isozyme at 71%, for PKp-isozyme at 39% the rate of ADP | Monoraphidium minutum | GTP + pyruvate | - |
? | |
| IDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | ITP + pyruvate | - |
? | |
| IDP + phosphoenolpyruvate | reaction with PKp-isozyme at 20%, with PKc-isozyme at 89% the rate of ADP | Monoraphidium minutum | ITP + pyruvate | - |
? | |
| UDP + phosphoenolpyruvate | reaction at about 70% the rate of ADP, PKc-isozyme | Monoraphidium minutum | UTP + pyruvate | - |
? | |
| UDP + phosphoenolpyruvate | less effective than ADP | Monoraphidium minutum | UTP + pyruvate | - |
? | |
| UDP + phosphoenolpyruvate | reaction at about 31% the rate of ADP, PKp-isozyme | Monoraphidium minutum | UTP + pyruvate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Monoraphidium minutum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
isozyme PKp | Monoraphidium minutum |
| 7 | - |
isozyme PKc | Monoraphidium minutum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8 | isozyme PKp, about half-maximal activity at pH 5.2 and pH 8 | Monoraphidium minutum |
| 5.9 | 8.2 | isozyme PKc, about half-maximal activity at pH 5.9 and pH 8.2 | Monoraphidium minutum |
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