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Literature summary for 2.7.1.40 extracted from
- Pyruvate kinases from human erythrocytes and liver (1982), Methods Enzymol., 90, 131-140.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-diphosphate | activation | Homo sapiens |  |
| D-fructose 1,6-diphosphate | major allosteric activator | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| L-alanine | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic properties | Homo sapiens | |
| additional information | - |
additional information | allosteric enzyme | Homo sapiens | |
| additional information | - |
additional information | kinetic properties of phosphorylated and dephosphorylated kinases | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
structure of liver and erythrocyte L-type kinases seems to be identical with an additional fragment present in erythrocytic enzyme | Homo sapiens |
| 60000 | - |
4 * 60000, L-type isozyme, SDS-PAGE | Homo sapiens |
| 60000 | - |
2 * 60000 + 2 * 57000-58000, most important of the "aged" isozymes, PKR2, SDS-PAGE, derived from erythroblast homotetramer by partial proteolysis and transformation into various active heterotetrameric forms with two partially proteolyzed subunits | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 330 | - |
pH 8.0, 30°C, erythrocyte enzyme | Homo sapiens |
| 420 | - |
liver enzyme, pH 8.0, 30°C | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, enzyme precipitate in ammonium sulfate solution, several years | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Homo sapiens | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, L-type isozyme, SDS-PAGE | Homo sapiens |
| tetramer | 2 * 60000 + 2 * 57000-58000, most important of the "aged" isozymes, PKR2, SDS-PAGE, derived from erythroblast homotetramer by partial proteolysis and transformation into various active heterotetrameric forms with two partially proteolyzed subunits | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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