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Literature summary for 2.7.1.40 extracted from

  • Flanders, L.E.; Schloen, L.H.; Sallach, H.J.
    Isozymes of pyruvate kinase from the grasfrog (1975), Methods Enzymol., 42C, 166-175.
No PubMed abstract available
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
D-fructose diphosphate activation Lithobates pipiens
D-fructose diphosphate cardiac and liver isozyme, together with phosphoenolpyruvate Lithobates pipiens

General Stability

General Stability Organism
glycerol, 50%, stabilizes Lithobates pipiens

Inhibitors

Inhibitors Comment Organism Structure
L-alanine
-
 Lithobates pipiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Lithobates pipiens

Organism

Organism UniProt Comment Textmining
Lithobates pipiens
-
 grass frog
-

Purification (Commentary)

Purification (Comment) Organism
major of 5 liver isozymes Lithobates pipiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Lithobates pipiens
-
muscle skeletal Lithobates pipiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lithobates pipiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
 Lithobates pipiens ATP + pyruvate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 assay temperature of cardiac and liver enzyme, value must not exceed 15°C Lithobates pipiens
20 21 muscle enzyme, assay at Lithobates pipiens