Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | substrate inhibition above 1 mM; substrate inhibition above 1 mM; substrate inhibition above 1 mM; substrate inhibition above 1 mM; substrate inhibition above 1 mM | Arabidopsis thaliana | |
EDTA | - |
Arabidopsis thaliana | |
GTP | FRK1 activity decreases by 35% activity in the presence of 1 mM GTP; FRK2 activity decreases by about 50% activity in the presence of 1 mM GTP; FRK3 activity decreases by about 50% activity in the presence of 1 mM GTP | Arabidopsis thaliana | |
additional information | no substrate inhibition by D-fructose (above 1 mM); no substrate inhibition by D-fructose (above 1 mM) | Arabidopsis thaliana | |
UTP | FRK1 activity decreases by 85% activity in the presence of 1 mM UTP; FRK2 activity decreases by 75% activity in the presence of 1 mM UTP; FRK3 activity decreases by 75% activity in the presence of 1 mM UTP | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
0.052 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
0.052 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
0.085 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
0.095 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
0.16 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
0.18 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
0.23 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
0.26 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
0.28 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
0.32 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
0.37 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
0.47 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
0.48 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Arabidopsis thaliana | to enter metabolism, fructose is phosphorylated by a fructokinase | ADP + D-fructose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O82616 | - |
- |
Arabidopsis thaliana | Q9C524 | - |
- |
Arabidopsis thaliana | Q9FLH8 | - |
- |
Arabidopsis thaliana | Q9LNE3 | - |
- |
Arabidopsis thaliana | Q9LNE4 | - |
- |
Arabidopsis thaliana | Q9M1B9 | - |
- |
Arabidopsis thaliana | Q9SID0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | to enter metabolism, fructose is phosphorylated by a fructokinase | Arabidopsis thaliana | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-fructose | no activity with ribose, xylose, fucose, glucose, tagatose or arabinose | Arabidopsis thaliana | ADP + D-fructose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At1g06020 | - |
Arabidopsis thaliana |
At1g66430 | - |
Arabidopsis thaliana |
At2g31390 | - |
Arabidopsis thaliana |
At3g59480 | - |
Arabidopsis thaliana |
At4g10260 | - |
Arabidopsis thaliana |
At5g51830 | - |
Arabidopsis thaliana |
Frk1 | - |
Arabidopsis thaliana |
Frk2 | - |
Arabidopsis thaliana |
Frk3 | ambiguous | Arabidopsis thaliana |
Frk4 | ambiguous | Arabidopsis thaliana |
FRK5 | ambiguous | Arabidopsis thaliana |
FRK6 | ambiguos | Arabidopsis thaliana |
FRK7 | ambiguous | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
1.5 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
4.8 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
4.8 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
10.3 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
10.3 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
11.1 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
11.1 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
12.8 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
12.8 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
14.2 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
14.2 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
14.3 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
14.3 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | to enter metabolism, fructose is phosphorylated by a fructokinase | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
120 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
270 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
270 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
273 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
800 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana |