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Literature summary for 2.7.1.4 extracted from

  • Caescu, C.I.; Vidal, O.; Krzewinski, F.; Artenie, V.; Bouquelet, S.
    Bifidobacterium longum requires a fructokinase (Frk; ATP:D-fructose 6-phosphotransferase, EC 2.7.1.4) for fructose catabolism (2004), J. Bacteriol., 186, 6515-6525.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.4

Inhibitors

Inhibitors Comment Organism Structure
ATP inhibitory above 12 mM Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.739
-
 D-fructose pH 6.5, 50°C Bifidobacterium longum
0.756
-
 ATP
-
 Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 x * 32500, calculated, x * 35000, SDS-PAGE Bifidobacterium longum
35000
-
 x * 32500, calculated, x * 35000, SDS-PAGE Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum
-
 induction by D-fructose, repression by D-glucose, expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Bifidobacterium longum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.84
-
 substrate D-fructose, pH 6.5, 50°C Bifidobacterium longum
1.8
-
 substrate ATP, pH 6.5, 50°C Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose highly specific for D-fructose Bifidobacterium longum ADP + D-fructose 6-phosphate
-
 ?
CTP + D-fructose 6% of activity with ATP Bifidobacterium longum CDP + D-fructose 6-phosphate
-
 ?
GTP + D-fructose 23% of activity with ATP Bifidobacterium longum GDP + D-fructose 6-phosphate
-
 ?
ITP + D-fructose 78% of activity with ATP Bifidobacterium longum IDP + D-fructose 6-phosphate
-
 ?
TTP + D-fructose 0.18% of activity with ATP Bifidobacterium longum TDP + D-fructose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 32500, calculated, x * 35000, SDS-PAGE Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bifidobacterium longum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6 40 gradual decrease of activtiy in this range Bifidobacterium longum
37 39 15 min, 70% residual activity Bifidobacterium longum
60
-
 15 min, complete loss of activity Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bifidobacterium longum

pH Range

pH Minimum pH Maximum Comment Organism
5 8 marked decrease in activity below and above Bifidobacterium longum