Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibition is dependent on fructose concentration | Solanum lycopersicum | |
D-fructose | above 0.5 mM | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
ATP | FKI, pH 8, 25°C | Solanum lycopersicum | |
0.131 | - |
D-fructose | FKI, pH 8, 25°C | Solanum lycopersicum | |
0.15 | - |
ATP | FKII, pH 8, 25°C | Solanum lycopersicum | |
0.201 | - |
D-fructose | FKII, pH 8, 25°C | Solanum lycopersicum | |
0.69 | - |
GTP | FKI, pH 8, 25°C | Solanum lycopersicum | |
0.9 | - |
GTP | FKII, pH 8, 25°C | Solanum lycopersicum | |
1.18 | - |
UTP | FKI, pH 8, 25°C | Solanum lycopersicum | |
1.51 | - |
UTP | FKII, pH 8, 25°C | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Solanum lycopersicum |
73000 | - |
gel filtration | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Solanum lycopersicum | - |
ADP + D-fructose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
400fold, FKI and FKII | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.2 | - |
FKII | Solanum lycopersicum |
25 | - |
FKI | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Solanum lycopersicum | ADP + D-fructose 6-phosphate | - |
? | |
GTP + D-fructose | - |
Solanum lycopersicum | GDP + D-fructose 6-phosphate | - |
? | |
UTP + D-fructose | - |
Solanum lycopersicum | UDP + D-fructose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
Solanum lycopersicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.39 | - |
D-fructose | FKII, pH 8, 25°C | Solanum lycopersicum | |
1.82 | - |
D-fructose | FKI, pH 8, 25°C | Solanum lycopersicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Solanum lycopersicum | 4 components, FKII, two-dimensional isoelectric focusing/SDS-PAGE | 6.55 | 6.07 |
Solanum lycopersicum | 2 components, FKI, two-dimensional isoelectric focusing /SDS-PAGE | 6.55 | 6.42 |