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Literature summary for 2.7.1.4 extracted from

  • Martinez-Barajas, E.; Randall, D.D.
    Purification and characterization of fructokinase from developing tomato (Lycopersicon esculentum) fruits (1996), Planta, 199, 451-458.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibition is dependent on fructose concentration Solanum lycopersicum
D-fructose above 0.5 mM Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
ATP FKI, pH 8, 25°C Solanum lycopersicum
0.131
-
D-fructose FKI, pH 8, 25°C Solanum lycopersicum
0.15
-
ATP FKII, pH 8, 25°C Solanum lycopersicum
0.201
-
D-fructose FKII, pH 8, 25°C Solanum lycopersicum
0.69
-
GTP FKI, pH 8, 25°C Solanum lycopersicum
0.9
-
GTP FKII, pH 8, 25°C Solanum lycopersicum
1.18
-
UTP FKI, pH 8, 25°C Solanum lycopersicum
1.51
-
UTP FKII, pH 8, 25°C Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Solanum lycopersicum
73000
-
gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Solanum lycopersicum
-
ADP + D-fructose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
400fold, FKI and FKII Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.2
-
FKII Solanum lycopersicum
25
-
FKI Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Solanum lycopersicum ADP + D-fructose 6-phosphate
-
?
GTP + D-fructose
-
Solanum lycopersicum GDP + D-fructose 6-phosphate
-
?
UTP + D-fructose
-
Solanum lycopersicum UDP + D-fructose 6-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5
-
Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.39
-
D-fructose FKII, pH 8, 25°C Solanum lycopersicum
1.82
-
D-fructose FKI, pH 8, 25°C Solanum lycopersicum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum lycopersicum 4 components, FKII, two-dimensional isoelectric focusing/SDS-PAGE 6.55 6.07
Solanum lycopersicum 2 components, FKI, two-dimensional isoelectric focusing /SDS-PAGE 6.55 6.42