Any feedback?
Literature summary for 2.7.1.4 extracted from
- Identification and properties of an inducible and highly specific fructokinase from Streptomyces violaceoruber (1972), Biochim. Biophys. Acta, 284, 414-420.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | - |
Streptomyces violaceoruber |  |
| MgADP- | competitive | Streptomyces violaceoruber | |
| MgATP2- | - |
Streptomyces violaceoruber | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
ATP | ATP in form of MgATP2- | Streptomyces violaceoruber | |
| 0.5 | - |
D-fructose | - |
Streptomyces violaceoruber | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ in activation | Streptomyces violaceoruber | |
| Mg2+ | activation by free Mg2+ | Streptomyces violaceoruber | |
| additional information | no activation by Mn2+ | Streptomyces violaceoruber | |
| additional information | no activation by Cd2+ | Streptomyces violaceoruber | |
| additional information | no activation by Cu2+ | Streptomyces violaceoruber |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | Streptomyces violaceoruber | inducible enzyme | ADP + D-fructose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces violaceoruber | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 400fold | Streptomyces violaceoruber |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Streptomyces violaceoruber | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces violaceoruber |
| 40 | - |
- |
Streptomyces violaceoruber |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in presence of Mg2+ and dithiothreitol, 25% loss of activity after 6 months | Streptomyces violaceoruber |
| 2°C, pH 7, in presence of Mg2+ and dithioerythritol, 25% loss of activity after 2 weeks | Streptomyces violaceoruber |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | specific for fructose | Streptomyces violaceoruber | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-fructose | inducible enzyme | Streptomyces violaceoruber | ADP + D-fructose 6-phosphate | - |
? | |
| CTP + D-fructose | 30% of the activity with ATP | Streptomyces violaceoruber | CDP + D-fructose 6-phosphate | - |
? | |
| dATP + D-fructose | 80% of the activity with ATP | Streptomyces violaceoruber | dADP + D-fructose 6-phosphate | - |
? | |
| GTP + D-fructose | 50% of the activity with ATP | Streptomyces violaceoruber | GDP + D-fructose 6-phosphate | - |
? | |
| ITP + D-fructose | 60% of the activity with ATP | Streptomyces violaceoruber | IDP + D-fructose 6-phosphate | - |
? | |
| UTP + D-fructose | 20% of the activity with ATP | Streptomyces violaceoruber | UDP + D-fructose 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | 10 | lengthy plateau in the alkaline zone | Streptomyces violaceoruber |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
1 h, 50% loss of activity | Streptomyces violaceoruber |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
MgATP2- | - |
Streptomyces violaceoruber | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
