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Literature summary for 2.7.1.4 extracted from

  • Doehlert, D.C.
    Fructokinases from developing maize kernels differ in their specificity for nucleoside triphosphates (1990), Plant Physiol., 93, 353-355.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
DTT, 10 mM, necessary to stabilize activity Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Zea mays
-
ADP + D-fructose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of UTP-dependent enzyme: CTP, GTP and UTP are more effective as phosphate donors than ATP, organism also has a nonspecific fructokinase and a third fairly specific activity for ATP Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
kernel
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
Zea mays ADP + D-fructose 6-phosphate
-
?
CTP + D-fructose 7.4times higher activity than with ATP Zea mays CDP + D-fructose 6-phosphate
-
?
GTP + D-fructose 3fold higher activity than with ATP Zea mays GDP + D-fructose 6-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Zea mays