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Literature summary for 2.7.1.4 extracted from

  • Thompson, J.; Sackett, D.L.; Donkersloot, J.A.
    Purification and properties of fructokinase I from Lactococcus lactis. Localization of scrK on the sucrose-nisin transposon Tn5306 (1991), J. Biol. Chem., 266, 22626-22633.
    View publication on PubMed
Search for more literature for 2.7.1.4

General Stability

General Stability Organism
no significant loss of activity after lyophilization and reconstitution of the enzyme Lactococcus lactis
stable after repeated freezing, at -20°C, and thawing Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 D-fructose pH 7.5, 22°C Lactococcus lactis
0.59
-
 ATP
-
 Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ can partially replace Mg2+ in activation Lactococcus lactis
Co2+ can partially replace Mg2+ in activation Lactococcus lactis
Fe2+ can partially replace Mg2+ in activation Lactococcus lactis
Mg2+ Km: 0.53 mM Lactococcus lactis
Mg2+ obligate requirement for divalent metal ion, maximal activity with 10 mM Mg2+, other cations can partially replace Mg2+: Co2+, Fe2+, Mn2+, Ni2+, Zn2+, Cd2+ Lactococcus lactis
Mn2+
-
 Lactococcus lactis
additional information no activation by Ca2+ Lactococcus lactis
additional information no activation by Cu2+ Lactococcus lactis
additional information no activation by Ba2+ Lactococcus lactis
additional information no activation by Sr2+ Lactococcus lactis
Ni2+ can partially replace Mg2+ in activation Lactococcus lactis
Zn2+ can partially replace Mg2+ in activation Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE after complete reduction with 2-mercaptoethanol Lactococcus lactis
44000
-
 gel filtration Lactococcus lactis
60000
-
 nondenaturing equilibrium ultracentrifugation Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose Lactococcus lactis
-
 ADP + D-fructose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 fructokinase I: induced specifically by growth on sucrose
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
112
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose
-
 Lactococcus lactis ADP + D-fructose 6-phosphate
-
 ?
ATP + D-mannose
-
 Lactococcus lactis ADP + D-mannose 6-phosphate
-
 ?
dATP + D-fructose
-
 Lactococcus lactis dADP + D-fructose 6-phosphate
-
 ?
GTP + D-fructose
-
 Lactococcus lactis GDP + D-fructose 6-phosphate
-
 ?
ITP + D-fructose
-
 Lactococcus lactis IDP + D-fructose 6-phosphate
-
 ?
additional information very low activity as phosphate donors: dGTP, TTP, XTP Lactococcus lactis ?
-
 ?
UTP + D-fructose
-
 Lactococcus lactis UDP + D-fructose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE after complete reduction with 2-mercaptoethanol Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Lactococcus lactis

pI Value

Organism Comment pI Value Maximum pI Value
Lactococcus lactis polypeptide 1, pre-cast Ampholine PAGplates at 10°C 5.9 5.6