Cloned (Comment) | Organism |
---|---|
expression of a His-tagged construct in Escherichia coli | Arabidopsis thaliana |
General Stability | Organism |
---|---|
enzyme stored with 3 mM ATP loses 50% of its activity after two freezing cycles. In presence of 50% v/v glycerol the enzyme loses 50% of its activity after 10 cycles. In the basic storage buffer, the enzyme loses 80% of its activity after a single freeze-thaw cycle | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
ATP | - |
Arabidopsis thaliana | |
0.4 | - |
L-homoserine | - |
Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | requires 40 mM K+ | Arabidopsis thaliana | |
Mn2+ | requires 3 mM | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-homoserine | Arabidopsis thaliana | formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants | ADP + O-phospho-L-homoserine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8L7R2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.09 | - |
L-homoserine-dependent ADP synthesis assay at pH 8.5 and 37°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-homoserine | - |
Arabidopsis thaliana | ADP + O-phospho-L-homoserine | - |
? | |
ATP + L-homoserine | formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants | Arabidopsis thaliana | ADP + O-phospho-L-homoserine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |