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Literature summary for 2.7.1.36 extracted from

  • Chen, Q.; Yan, J.; Meng, X.; Xu, F.; Zhang, W.; Liao, Y.; Qu, J.
    Molecular cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and mevalonate kinase genes involved in terpene trilactone biosynthesis from Ginkgo biloba (2017), Molecules, 22, 74 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.36

Cloned(Commentary)

Cloned (Comment) Organism
gene MVK, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression and functional complementation of GbMVK in haploid disrupted MVK-deficient Saccharomyces cerevisiae strain DELTAerg12. Quantitative RT-PCR enzyme expression analysis Ginkgo biloba

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Ginkgo biloba 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Ginkgo biloba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Ginkgo biloba
-
 ADP + (R)-5-phosphomevalonate
-
 ?

Organism

Organism UniProt Comment Textmining
Ginkgo biloba A0A1S6KJR4 plant samples obtained from 18-year-old trees of Ginkgo biloba growing in the Ginkgo garden of Yangtze University, Hubei, China (around N30.35, E112.14)
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Ginkgo biloba
-
additional information GbMVK is highly expressed in leaves and roots, tissue expression pattern analysis, overview. Quantitative RT-PCR enzyme expression analysis Ginkgo biloba
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root
-
 Ginkgo biloba
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seedling
-
 Ginkgo biloba
-
stem
-
 Ginkgo biloba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
 Ginkgo biloba ADP + (R)-5-phosphomevalonate
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, about, sequence calculation Ginkgo biloba
More the structure of GbMVK monomer contains a ribosomal protein S5 2-type (8-230), a GHMP kinase N-terminal domain (140-220), and a GHMP kinase C-terminal domain (236-390). Ile146-Ala157 is an ATP-binding conserved site Ginkgo biloba

Synonyms

Synonyms Comment Organism
GbMVK
-
 Ginkgo biloba
MVK
-
 Ginkgo biloba

pI Value

Organism Comment pI Value Maximum pI Value
Ginkgo biloba sequence calculation
-
 5.71

Expression

Organism Comment Expression
Ginkgo biloba methyl jasmonate and salicylic acid increase the expression level of GbMVK and enhance the terpene trilactones (TTLs) production in Ginkgo biloba seedlings up

General Information

General Information Comment Organism
evolution GbAACT and GbMVK are highly homologous to AACT genes and MVK genes from other plant species. The structure of GbMVK monomer contains a ribosomal protein S5 2-type (8-230), a GHMP kinase N-terminal domain (140-220), and a GHMP kinase C-terminal domain (236-390). Ile146-Ala157 is an ATP-binding conserved site. Molecular evolution analysis and phylogenetic analysis, overview Ginkgo biloba
metabolism ginkgolides and bilobalides, collectively termed terpene trilactones (TTLs), are terpenoids that form the main active substance of Ginkgo biloba. Terpenoids in the mevalonate (MVA) biosynthetic pathway include acetyl-CoA C-acetyltransferase (AACT) and mevalonate kinase (MVK) as core enzymes, pathways overview. TTL content in Ginkgo biloba can be enhanced by upregulating the transcript levels of some of these genes Ginkgo biloba
physiological function enzyme GbMVK mediates the conversion of mevalonate to mevalonate phosphate and is involved in biosynthesis of ginkgolides and bilobalides, termed terpene trilactones (TTLs), in Ginkgo biloba. GbAACT and GbMVK are functional genes in the cytosolic mevalonate (MVA) biosynthesis pathway Ginkgo biloba