Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methanosarcina mazei |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.32 M MgCl2, 0.08 M bis-Tris pH 5.5, 16% (w/v) PEG 3350, at 18°C | Methanosarcina mazei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE | Methanosarcina mazei |
33600 | - |
2 * 33600, His-tagged protein, calculated from amino acid sequence | Methanosarcina mazei |
64600 | - |
gel filtration | Methanosarcina mazei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | Q8PW39 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA bead column chromatography, Resource 15Q column chromatography, and Superdex 200 gel filtration | Methanosarcina mazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | - |
Methanosarcina mazei | ADP + (R)-5-phosphomevalonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 33000, SDS-PAGE | Methanosarcina mazei |
homodimer | 2 * 33600, His-tagged protein, calculated from amino acid sequence | Methanosarcina mazei |
Synonyms | Comment | Organism |
---|---|---|
MVK | - |
Methanosarcina mazei |