Any feedback?
Literature summary for 2.7.1.36 extracted from
- Staphylococcus aureus mevalonate kinase: isolation and characterization of an enzyme of the isoprenoid biosynthetic pathway (2004), J. Bacteriol., 186, 61-67.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
| expressed in Escherichia coli BL21(DE3) | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-phosphomevalonate | product inhibition, non-competitive with ATP and mevalonate | Staphylococcus aureus |  |
| farnesyl diphosphate | feedback inhibition, competitive with ATP | Homo sapiens | |
| farnesyl diphosphate | feedback inhibition, competitive with ATP | Rattus norvegicus | |
| farnesyl diphosphate | feedback inhibition, competitive with ATP | Staphylococcus aureus | |
| farnesyl thiodiphosphate | feedback inhibition, competitive with ATP | Homo sapiens | |
| farnesyl thiodiphosphate | feedback inhibition, competitive with ATP | Rattus norvegicus | |
| farnesyl thiodiphosphate | feedback inhibition, competitive with ATP | Staphylococcus aureus | |
| mevalonate | substrate inhibition at millimolar concentrations, 50% inhibition at 2.88 mM | Staphylococcus aureus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.041 | - |
R,S-mevalonate | pH 7.5, 30°C | Staphylococcus aureus | |
| 0.339 | - |
ATP | pH 7.5, 30°C | Staphylococcus aureus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Staphylococcus aureus | 5829 | - |
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Staphylococcus aureus | |
| Mn2+ | 25% of the activity with Mg2+ | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000, SDS-PAGE and gel filtration | Staphylococcus aureus |
| 34500 | - |
gel filtration | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-mevalonate | Rattus norvegicus | involved in cholesterol synthesis | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | Homo sapiens | involved in cholesterol synthesis, decreased enzyme activity involved in mevalonic aciduria and hyperimmunoglobulin D syndrome | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | Staphylococcus aureus | involved in isopentenyl diphosphate synthesis | ADP + (R)-5-phosphomevalonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.4 | - |
purified enzyme, pH 7.5, 30°C | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-mevalonate | - |
Staphylococcus aureus | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | - |
Homo sapiens | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | - |
Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | involved in cholesterol synthesis | Rattus norvegicus | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | involved in cholesterol synthesis, decreased enzyme activity involved in mevalonic aciduria and hyperimmunoglobulin D syndrome | Homo sapiens | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + (R)-mevalonate | involved in isopentenyl diphosphate synthesis | Staphylococcus aureus | ADP + (R)-5-phosphomevalonate | - |
? | |
| ATP + mevalonate | - |
Staphylococcus aureus | ADP + phosphomevalonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, SDS-PAGE and gel filtration | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Staphylococcus aureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Staphylococcus aureus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000029 | - |
farnesyl thiodiphosphate | pH 7.5, 30°C | Homo sapiens | |
| 0.000035 | - |
farnesyl diphosphate | pH 7.5, 30°C | Homo sapiens | |
| 0.00035 | - |
farnesyl diphosphate | pH 7.5, 30°C | Rattus norvegicus | |
| 0.00047 | - |
farnesyl thiodiphosphate | pH 7.5, 30°C | Rattus norvegicus | |
| 0.045 | - |
farnesyl thiodiphosphate | pH 7.5, 30°C | Staphylococcus aureus | |
| 0.046 | - |
farnesyl diphosphate | pH 7.5, 30°C | Staphylococcus aureus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
