BRENDA - Enzyme Database
show all sequences of 2.7.1.36

Post-translational regulation of mevalonate kinase by intermediates of the cholesterol and nonsterol isoprene biosynthetic pathways

Hinson, D.D.; Chambliss, K.L.; Toth, M.J.; Tanaka, R.D.; Gibson, K.M.; J. Lipid Res. 38, 2216-2223 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
3,3-dimethylallyl diphosphate
-
Homo sapiens
dolichol phosphate
-
Homo sapiens
farnesol
-
Homo sapiens
farnesyl diphosphate
-
Homo sapiens
geranyl diphosphate
-
Homo sapiens
geranylgeranyl diphosphate
-
Homo sapiens
isopentenyl diphosphate
-
Homo sapiens
additional information
not inhibited by R-mevalonate 5-diphosphate, geraniol and dolichol up to 0.084, 0.226 and 0.68 mM, respectively
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
(R,S)-mevalonate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.44
-
ATP
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42500
-
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + mevalonate
Homo sapiens
part of the cholesterol and nonsterol isoprene biosynthetic pathway
ADP + phosphomevalonate
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant mevalonat kinase-glutathione S-transferase fusion protein, glutathione Sepharose beads
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + mevalonate
-
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
ATP + mevalonate
part of the cholesterol and nonsterol isoprene biosynthetic pathway
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
Subunits
Subunits
Commentary
Organism
?
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000104
-
farnesyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.016
-
isopentenyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.02
-
3,3-dimethylallyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.059
-
geranylgeranyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.072
-
farnesol
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.083
-
dolichol phosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.116
-
geranyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,3-dimethylallyl diphosphate
-
Homo sapiens
dolichol phosphate
-
Homo sapiens
farnesol
-
Homo sapiens
farnesyl diphosphate
-
Homo sapiens
geranyl diphosphate
-
Homo sapiens
geranylgeranyl diphosphate
-
Homo sapiens
isopentenyl diphosphate
-
Homo sapiens
additional information
not inhibited by R-mevalonate 5-diphosphate, geraniol and dolichol up to 0.084, 0.226 and 0.68 mM, respectively
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000104
-
farnesyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.016
-
isopentenyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.02
-
3,3-dimethylallyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.059
-
geranylgeranyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.072
-
farnesol
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.083
-
dolichol phosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.116
-
geranyl diphosphate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
(R,S)-mevalonate
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
0.44
-
ATP
pH 7.0, 25°C, recombinant mevalonate kinase
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42500
-
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + mevalonate
Homo sapiens
part of the cholesterol and nonsterol isoprene biosynthetic pathway
ADP + phosphomevalonate
-
Homo sapiens
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant mevalonat kinase-glutathione S-transferase fusion protein, glutathione Sepharose beads
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + mevalonate
-
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
ATP + mevalonate
part of the cholesterol and nonsterol isoprene biosynthetic pathway
641447
Homo sapiens
ADP + phosphomevalonate
-
641447
Homo sapiens
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42500, recombinant mevalonate kinase, SDS-PAGE
Homo sapiens
Other publictions for EC 2.7.1.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739684
Ferreira
Unique behavior of Trypanosoma ...
Trypanosoma cruzi, Trypanosoma cruzi CL Brener
Sci. Rep.
6
24610
2016
-
-
1
-
-
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3
-
1
2
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7
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1
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1
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1
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1
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2
1
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1
1
-
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-
738399
Kumari
Biochemical characterization o ...
Bacopa monnieri
Int. J. Biol. Macromol.
72
776-783
2015
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1
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-
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2
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1
1
1
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3
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1
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1
1
1
1
1
2
1
1
1
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1
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2
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1
1
1
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1
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1
1
1
1
1
2
1
1
1
-
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-
-
-
2
2
739434
Nyati
Negative feedbacks by isopreno ...
Aedes aegypti
PLoS ONE
10
e0143107
2015
-
-
1
-
-
-
4
2
-
3
1
1
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4
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1
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3
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4
1
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1
1
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3
1
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1
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4
3
2
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3
1
1
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1
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3
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-
4
1
-
-
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1
1
-
-
1
-
-
-
-
-
-
722571
Nishimura
Biochemical evidence supportin ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
J. Biochem.
153
415-420
2013
-
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1
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1
2
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6
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1
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4
1
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1
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1
2
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1
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4
1
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-
721225
Zhuang
Crystallization and preliminar ...
Methanosarcina mazei
Acta Crystallogr. Sect. F
68
1560-1563
2012
-
-
1
1
-
-
-
-
-
-
3
-
-
3
-
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1
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1
1
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1
1
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-
721347
Primak
Characterization of a feedback ...
Methanosarcina mazei
Appl. Environ. Microbiol.
77
7772-7778
2011
-
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1
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1
2
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2
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4
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1
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1
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1
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1
1
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1
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707737
Kudoh
Mevalonate analogues as substr ...
Streptococcus pneumoniae
Bioorg. Med. Chem.
18
1124-1134
2009
-
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1
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2
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1
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3
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2
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1
1
-
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-
708647
Takase
Parathyroid hormone upregulate ...
Mus musculus
Horm. Metab. Res.
41
861-865
2009
-
-
-
-
-
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1
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3
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1
2
2
1
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709706
Steichen
A clinical criterion to exclud ...
Homo sapiens
J. Rheumatol.
36
1677-1681
2009
-
1
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1
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2
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-
1
1
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-
690941
Fu
Biochemical and structural bas ...
Homo sapiens, Rattus norvegicus
Biochemistry
47
3715-3724
2008
-
-
2
2
16
-
2
18
-
-
-
-
-
6
-
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-
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-
-
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2
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9
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2
-
2
16
-
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2
9
18
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-
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2
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691461
Kuijk
HMG-CoA reductase inhibition i ...
Homo sapiens
Blood
112
3563-3573
2008
-
1
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2
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1
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1
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-
691885
Topaloglu
Hyperimmunoglobulinemia D and ...
Homo sapiens
Clin. Rheumatol.
27
1317-1320
2008
-
1
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1
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1
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692079
Ikeda
Effect of estrogen on the expr ...
Rattus norvegicus
Endocrinology
149
1524-1533
2008
-
1
-
-
-
-
2
-
-
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2
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1
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1
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692190
Sornsakrin
B cell cytopenia in two brothe ...
Homo sapiens
Eur. J. Pediatr.
168
825-831
2008
-
1
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-
1
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2
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692301
Nair
Regulation of luteinizing horm ...
Rattus norvegicus
FEBS J.
275
3397-3407
2008
-
-
1
-
9
-
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3
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1
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9
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693507
van der hilst
Lovastatin inhibits formation ...
Homo sapiens
J. Leukocyte Biol.
83
1295-1299
2008
-
1
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1
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2
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1
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693997
Anthony
Optimization of the mevalonate ...
Escherichia coli
Metab. Eng.
11
13-19
2008
-
1
1
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1
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1
1
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694550
Marcuzzi
Natural isoprenoids are able t ...
Mus musculus
Pediatr. Res.
64
177-182
2008
-
1
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4
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1
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672817
Sgraja
Structure, substrate recogniti ...
Leishmania major, Trypanosoma brucei
BMC Struct. Biol.
7
20
2007
-
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2
1
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1
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2
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7
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1
1
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1
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2
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1
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1
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4
-
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3
4
2
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1
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2
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1
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1
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2
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1
-
-
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1
-
-
-
-
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-
-
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-
677036
Andreassi
Crystal structure of the Strep ...
Streptococcus pneumoniae
Protein Sci.
16
983-989
2007
-
-
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-
-
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-
3
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-
690444
Kone-Paut
Autoinflammatory gene mutation ...
Homo sapiens
Ann. Rheum. Dis.
66
832-834
2007
-
1
-
-
-
-
-
-
-
-
-
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-
3
-
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-
-
-
-
690694
Murphy
Regulation by SREBP-2 defines ...
Homo sapiens, Mus musculus
Biochem. Biophys. Res. Commun.
355
359-364
2007
1
-
-
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-
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5
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691055
Chu
Effect of a disulfide bond on ...
Methanocaldococcus jannaschii, Rattus norvegicus
Biochim. Biophys. Acta
1774
1571-1581
2007
-
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7
-
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17
-
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5
-
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1
1
3
9
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691939
Schirra
Androgen influence on choleste ...
Mus musculus
Curr. Eye Res.
32
393-398
2007
1
-
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3
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-
-
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-
-
-
-
693486
Breton Martinez
Mevalonic aciduria: report of ...
Homo sapiens
J. Inherit. Metab. Dis.
30
829
2007
-
1
-
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2
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-
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-
-
-
-
-
693487
Hager
Deletion of a single mevalonat ...
Mus musculus
J. Inherit. Metab. Dis.
30
888-895
2007
-
-
-
-
1
-
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-
-
-
-
-
-
6
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-
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-
-
694182
Wang
Ribonucleic acid binding prote ...
Homo sapiens
Mol. Endocrinol.
21
2233-2241
2007
-
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1
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1
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1
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-
-
-
694552
Nevyjel
Diagnostics and therapeutic in ...
Homo sapiens
Pediatrics
119
e523-e527
2007
-
1
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1
-
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-
-
-
-
-
-
-
695100
Ammouri
Diagnostic value of serum immu ...
Homo sapiens
Rheumatology
46
1597-1600
2007
-
1
-
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-
-
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2
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-
-
-
-
695101
Lequerre
Mevalonate kinase deficiency s ...
Homo sapiens
Rheumatology
46
1860-1862
2007
-
1
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1
-
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-
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
676284
Qiu
Bifunctional inhibitors of mev ...
Rattus norvegicus
Org. Lett.
8
1013-1016
2006
-
-
-
-
-
-
10
-
-
1
-
1
-
1
-
-
-
-
-
-
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2
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1
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9
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9
10
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2
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-
-
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-
-
-
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677225
Tang
Mevalonate kinase activity dur ...
Pinus strobus
Tree Physiol.
26
195-200
2006
-
-
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-
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-
-
-
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-
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1
-
4
-
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4
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4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673439
Hoffmann
Identification of a novel meva ...
Homo sapiens
Eur. J. Hum. Genet.
13
510-512
2005
-
-
1
-
2
-
-
-
-
1
-
1
-
2
-
-
-
-
-
1
-
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1
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1
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1
-
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
662019
Voynova
Staphylococcus aureus mevalona ...
Homo sapiens, Rattus norvegicus, Staphylococcus aureus
J. Bacteriol.
186
61-67
2004
-
-
3
-
-
-
8
2
3
2
2
3
-
6
-
-
1
-
-
-
1
-
7
1
-
-
-
-
1
1
-
-
6
-
-
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-
3
-
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-
8
6
2
3
2
2
3
-
-
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1
-
-
1
-
7
1
-
-
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-
1
1
-
-
-
-
-
-
-
-
662499
Hogenboom
Mevalonate kinase is a cytosol ...
Homo sapiens
J. Cell Sci.
117
631-639
2004
-
-
1
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
3
-
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-
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3
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2
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-
-
-
-
-
-
-
663334
Hedl
Enterococcus faecalis mevalona ...
Enterococcus faecalis
Protein Sci.
13
687-693
2004
-
1
1
-
-
-
2
8
1
4
3
1
-
3
-
-
1
1
-
-
1
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7
2
1
1
-
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1
1
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1
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1
1
-
-
-
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2
1
8
1
4
3
1
-
-
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1
-
-
1
-
7
2
1
1
-
-
1
1
-
-
-
-
-
-
-
-
641458
Chu
Cloning, expression, and purif ...
Rattus norvegicus
Protein Expr. Purif.
27
165-170
2003
-
-
1
-
-
-
-
2
-
-
2
-
-
5
-
-
1
-
-
-
1
1
1
1
-
-
-
-
1
1
-
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1
-
-
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2
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2
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1
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-
1
1
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
641459
Chu
Expression and purification of ...
Methanocaldococcus jannaschii
Protein Expr. Purif.
30
210-218
2003
-
-
-
-
4
-
-
12
-
-
-
-
-
3
-
-
-
-
-
-
-
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1
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4
-
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12
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663293
Chu
Expression, purification, and ...
Rattus norvegicus
Protein Expr. Purif.
32
75-82
2003
-
-
1
-
3
-
4
6
1
-
1
1
-
5
-
-
1
-
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
3
-
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4
-
6
1
-
1
1
-
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1
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641456
Houten
Temperature dependence of muta ...
Homo sapiens
Hum. Mol. Genet.
11
3115-3124
2002
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
2
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1
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
641457
Fu
The structure of a binary comp ...
Rattus norvegicus
J. Biol. Chem.
277
18134-18142
2002
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
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-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727840
Yang
Structure of the Methanococcus ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Biol. Chem.
277
9462-9467
2002
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641454
Rios
Characterization of mevalonate ...
Homo sapiens
Biochim. Biophys. Acta
1531
165-168
2001
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
641455
Cho
Investigation of invariant ser ...
Homo sapiens
J. Biol. Chem.
276
12573-12578
2001
-
-
-
-
4
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641451
Schulte
Purification and characterizat ...
Catharanthus roseus
Arch. Biochem. Biophys.
378
287-298
2000
-
-
-
-
-
-
3
2
-
4
3
1
-
2
-
-
1
-
-
1
1
-
2
1
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
2
-
4
3
1
-
-
-
1
-
1
1
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
641452
Schulte
-
Mevalonate kinase activity in ...
Arabidopsis thaliana, Catharanthus roseus, Homo sapiens, Nepeta cataria, Parthenium argentatum, Rattus norvegicus, Saccharomyces cerevisiae, Spinacia oleracea
Plant Sci.
150
59-69
2000
-
-
2
-
-
-
-
-
-
-
1
-
-
8
-
-
-
-
-
7
-
-
8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
7
-
-
8
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641453
Houten
Biochemical and genetic aspect ...
Homo sapiens, Methanocaldococcus jannaschii, Rattus norvegicus, Sus scrofa
Biochim. Biophys. Acta
1529
19-32
2000
-
-
1
-
10
-
4
7
3
-
1
-
-
5
-
-
-
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
-
10
-
-
4
4
7
3
-
1
-
-
-
-
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641450
Huang
Overexpression, purification, ...
Methanocaldococcus jannaschii
Protein Expr. Purif.
17
33-40
1999
-
-
1
-
-
-
2
2
-
-
2
1
-
3
-
-
1
-
-
-
1
-
2
1
1
1
3
-
1
1
-
-
-
2
-
-
-
1
-
-
-
-
-
2
-
2
-
-
2
1
-
-
-
1
-
-
1
-
2
1
1
1
3
-
1
1
-
2
-
-
-
-
-
-
641449
Bishop
Characterization of the mevalo ...
Homo sapiens
Biochem. Biophys. Res. Commun.
242
518-524
1998
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
641447
Hinson
Post-translational regulation ...
Homo sapiens
J. Lipid Res.
38
2216-2223
1997
-
-
1
-
-
-
8
2
-
-
1
1
-
3
-
-
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-
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-
-
-
641448
Potter
Identification and functional ...
Rattus norvegicus
J. Biol. Chem.
272
5741-5746
1997
-
-
1
-
1
-
-
4
-
-
2
-
-
4
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
698708
Potter
Identification of catalytic re ...
Homo sapiens
J. Biol. Chem.
272
25449-25454
1997
-
-
1
-
9
-
-
12
-
-
2
-
-
2
-
-
1
1
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1
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-
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641446
Graef
Enzymic properties of overexpr ...
Homo sapiens
Virology
208
696-703
1995
-
-
-
-
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2
-
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-
4
-
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-
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641439
Tanaka
Purification and regulation of ...
Rattus norvegicus
J. Biol. Chem.
265
2391-2398
1990
-
-
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-
-
1
1
2
-
-
2
1
-
2
-
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2
1
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1
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641432
Porter
Mevalonate kinase ...
Agave americana, Citrus sp., Cucumis melo, Cucurbita pepo, Hevea brasiliensis, Kalanchoe crenata, Oryctolagus cuniculus, Phaseolus vulgaris, Pinus pinaster, Pinus radiata, Rattus norvegicus, Saccharomyces cerevisiae, Sarcophaga bullata, Staphylococcus aureus, Sus scrofa
Methods Enzymol.
110
71-78
1985
-
-
-
-
-
-
8
-
-
15
1
-
-
15
-
-
1
1
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12
1
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16
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1
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12
1
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16
-
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1
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641441
Soong Lee
Phosphorothioate analogues of ...
Sus scrofa
Biochim. Biophys. Acta
787
131-137
1984
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21
-
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21
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1
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4
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641436
Soong Lee
An improved purification proce ...
Rattus norvegicus, Sus scrofa
Biochim. Biophys. Acta
747
215-224
1983
3
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1
1
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2
-
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3
-
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2
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2
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11
2
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641433
Soler
Hog liver mevalonate kinase: i ...
Sus scrofa
Int. J. Biochem.
10
931-935
1979
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2
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641440
Suarez
-
Properties and partial purific ...
Agave americana
Phytochemistry
16
661-665
1977
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1
1
2
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2
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1
1
4
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1
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641444
Suarez
-
Mevalonate kinase from Pinus p ...
Pinus pinaster
Phytochemistry
13
1059-1063
1974
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2
2
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1
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1
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2
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1
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1
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641434
Shewry
-
Properties of castor bean meva ...
Ricinus communis
Plant Sci. Lett.
1
473-477
1973
-
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2
-
1
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3
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641438
Gray
Mevalonate kinase in green lea ...
Phaseolus vulgaris
Biochem. J.
133
335-347
1973
1
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10
5
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2
1
-
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2
-
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6
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1
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10
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3
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6
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1
1
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-
-
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-
641442
Garcia-Peregrin
Isolation of two fractions wit ...
Agave americana, Pinus pinaster
FEBS Lett.
30
15-17
1973
-
-
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-
-
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-
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-
-
4
-
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-
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4
-
-
2
-
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-
641445
Gray
An assessment of some molecula ...
Cucumis melo, Gallus gallus, Hevea brasiliensis, Oryctolagus cuniculus, Phaseolus vulgaris, Saccharomyces cerevisiae
Arch. Biochem. Biophys.
159
458-462
1973
-
-
-
-
-
-
-
-
-
-
6
-
-
8
-
-
5
-
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5
-
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6
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5
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6
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-
-
641437
Gray
The inhibition of plant mevalo ...
Cucumis melo, Hevea brasiliensis, Phaseolus vulgaris, Saccharomyces cerevisiae
Biochim. Biophys. Acta
279
290-296
1972
-
-
-
-
-
-
16
4
-
-
-
-
-
5
-
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2
4
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4
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16
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16
16
4
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2
4
-
4
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641435
Goodfellow
-
Mevalonate kinase from the lar ...
Sarcophaga bullata
Insect Biochem.
1
271-282
1971
-
-
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-
-
2
-
-
1
-
-
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-
-
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-
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-
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1
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-
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-
-
-
-
-
-
-
-
641443
Williamson
The formation of 5-phosphomeva ...
Hevea brasiliensis
Biochem. J.
96
862-871
1965
1
-
-
-
-
-
7
2
-
6
-
-
-
2
-
-
1
-
-
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5
-
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-
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-
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7
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1
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5
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1
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2
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1
1
1
-
-
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-
-
-
-
641431
Markley
-
Mevalonic kinase in rabbit liv ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
47
327-335
1961
2
-
-
-
-
1
1
1
-
2
-
-
-
1
-
-
1
-
-
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1
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3
-
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1
1
3
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1
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1
-
-
-
-
-
-
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-
641430
Tchen
Mevalonic kinase: purification ...
Saccharomyces cerevisiae
J. Biol. Chem.
233
1100-1103
1958
1
-
-
-
-
-
1
-
-
6
-
-
-
1
-
-
1
-
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4
-
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1
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6
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1
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1
4
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1
1
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